多溴联苯醚对鲫鱼组织DNA损伤及p53蛋白表达的影响.pdf

农业环境科学学报201130(9):1836-18
Joumal of Agro-environment Science
多溴联苯醚对鲫鱼组织DNA损伤及p53蛋白表达的影向
吴伟12,程建宏,陈家长2,聂风琴2
(1中国水产科学研究院淡水渔业研究中心,中国水产科学研究院内陆渔业生态环境与资源重点开放实验室,江苏无锡214081;
2.南京农业大学渔业学院,江苏无媧214081)
摘要:以鯽鱼( Carassius auratus)为试验材料,研究了经不同浓度的2,22,4,4'-四溴联苯醚(PBDE-47)和十溴联苯醚(PBDEー
209)暴露后,鯽鱼体内DNA损伤产物8-羟基脱氧乌苷(8-OHdC)的含量及p53蛋白的水平。结果表明,采用浓度为0.10-5.00mg
L的PBDE-47和10.0-560.0mg·L的PBDE-209处理鲫鱼20d,除了0.10mg? L+ PBDE-47和10.0mg?L1PBDE-209浓度组的8-
OHdG和p53蛋白含量与对照组相比无显著差异(P0.05),其余各浓度组的8-OHdG和p53蛋白含量均随PBDE-47和PBDF-209
浓度增加而逐渐上升,呈显著的相关关系(P<001),且8-0HdG和p53蛋白之间也呈显著的正相关。说明PBDE-47和PBDE-209
对鲫鱼组织的DNA产生了损伤,具有遗传毒性影响。
关键词:多溴联苯醚;鲫鱼;DNA;损伤;p53蛋白
中图分类号:X503.225文献标志码:A文章编号:1672-2043(201109-1836-06
Effects of Polybrominated Dip enyl E hers on DNA Damage and p53 Protein Expression in Carassius auratus
linn
WU Wei, QU Jian-hong, CHEN Jia-zhang2, NIE Feng-qin
( 1. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences; Key Laboratory of Inland Fishery Eco-environment and Re-
source Chinese Academy of F sey enes Wx 214081, hina ishy College Nanjing cule Univerity, Wuxi 214081, China)
Abstract: Persistent or anic pollutants(POPS )has a long-term threat to different biota, including aquatic animals, mammals, especially hu-
mans due to their oma i ed ee n f hn, e study on the oxic ffect of POP o aquatic animals was evidently important and co-
cemed. This paper d cussed he vaia on Damage prduct 8-H x -esox uan ne(8-ohdg)and p53 protein to Cassius
wat in exposed o poly m ted d pen l the(pde-47 and PDE-209) where the exposed dose of PBDE-47 and PBDE
209 ran ed om 1 o 5. 0 mg L an 10.0 o 50.0 mg espetivel The ls shw h Dnaamageprut8-ohdgand p3
protein were generated ea n al amen as e or 0 a xe he OI mg in PBDE-47 and 10.0 in PBDE-209
The variation of 8- H G and p m ss when x PBDE-47 and PDE2 were s cantly do -depend
(P<0.01 ) resectively, and he a at on of H was a ly melted with e a on of p53 in a w. The ca
edthat,thegenotoicefect,characteriredbyDNA-damagepoducL,suchas8-0HdGandp53poteininCuasisouratuswhenexpogedto
PBDE-47andPBDE-209werepnodued.Thepaperprovidedmoreseienitedetalstotheresearchonthetoxicefectrespeilgenotoic
effect of POPS o aquatic anima, with a result to recognize, ontrol and eliminate POPS in different environmental medium.
Keywords polybrominated dip en] ether Cassius atus; DNA; damage; p53 protein
多溴联苯醚( polybrominated diphenyl ethers,PB-质稳定、可随食物链生物富集和放大,是一种持久性
DEs)属于溴系阻燃剂的一种,应用广泛,具有一定有机污染物四。自1981年首次在瑞典的梭鱼、鳗鲡中
的挥发性,可随大气长距离迁移,且亲脂性强、化学性检出后, PBDES已被发现在多种环境介质、人体和生
物材料中广泛存在,且含量呈逐年增加的趋势。PB
收日期:2011-02-24
DEs已被认为是普遍存在的环境污染物,对其环境问
甚金项目:中央级公益性科研院所基本科研业务费专项资金项目
(2007JBFB02);中国水产科学研究院内陆渔业生态环境与
题的研究成为当前环境科学的一大热点。迄今的研究
资源重点开放实验室开放课题(YM2007-10)
结果发现, PBDES对生物体的神经系统、甲状腺、肝和
作者简介:吴伟(1967-),男,研究员,主要从事污染生态学及环境
肾的影响较为明显。在 PBDE&中,2,2',4,4'-四溴
毒理学的研究。E-mail:wuw@ire.cni;wuwei@263,net
联苯醚(PBDE-47)是分布最广、生物材料中含量最
万方数据