生物复混肥对土壤微生物功能多样性及土壤酶活性的影响.pdf

农业环境科学学报2012,31(6:1153-1159
Joumal of Agro-envimonment Science
生物复混肥对土壤微生物功能多样性及
士壤酶活性的影响
邵丽1,谷洁,张社奇,高华2,秦清军2
1.西北农林科技大学理学院,映西杨凌712100;2.西北农林科技大学资源环境学院,陕西杨凌712100)
摘要;在温室盆栽条件下,采用 Biolog微平板技术,研究了玉米施用等养分量的无机肥、有机无机复混肥、生物复混肥后土微生
物群落功能多样性及十壤晦活性的动态变化。结果表明,生物复混肥处理的微生物群落平均色变化率(AWCD)、微生物群落
Shannan指数(F)、丰富度指数(S)和 Shannon均匀度指数(E)均为最高;微生物群落主成分分析表明,不同施肥处理上壤微生物群
落碳源利用特征有一定差异.PCL将生物复混肥与其他处理明显区分,生物复混肥处理分布在PCI的正方向,其他处理分布在PC
的负方向;起分异作用的主要碳源有糖类、羧酸类和氨棊酸类;土壤蔗糖酶、脲酶活性均以生物复混肥处理最高,分别为72.74mg
glueose"gt-(24h)和1.15 mg Niy~N-g'-(3h)。研究表明,生物复混肥的施用比等养分量的有机无机复混肥处理能显著提高土
壤徵生物群落碳源利用率、微生物群落的富度和功能多样性,增强上壤蔗糖和酶活性
关键词:生物复混肥;功能多样性;壤微生物群落;土壤酶活性
中图分类号:S1441文献标志码:A文章编号:1672-2043(2012)06~-1153-07
Effects of Bio-compound Fertilizer on Corn Soil Microbial Community and Enzyme Activities
SHA0 Li, GU Jie", ZHANG She-qil. GAO Hua', QIN (ing-jun
1 College of Scienees, Northwest A &F Univemity. Yan ling 712100, China; College of Resourees and Environment, Northwest A&F
University, Yangling 712100, China)
Abstract: Fei zaon ul i un l py cal and g pre ies By e biolg micr-plate technique, this study investi-
ga ed the effecls of bio-ompound fertiliz r, organic-in an fe, inor anic fe ilizer at the same mutr nt mount of N, P and K on
functional diversities of soil microbe communities and il enzyme acti ities under on potted experiment in greenhouse. The substrates of
Biolog ECO plate were composed of six pes of su stan s ao ates, carboxylic acids, amino acids, polymer, amines and aromatic
cemicals)Prineipal oment analysis was used o sw e ference utili tion of substrates by soil microbes. The results showed that
the Average Well-color Developments(A CD ) Shannon index(H Richmess index S)and Shanon evenness index(E)of microbe com-
munities appeared the highest in bio-compound fertilizer treatment. The principal component analysis(PCA) showed that the carbon source
use pattems of the sil mice comunities diered o a ain xtent under ifer er lization ramen s and the bi-mund fertili-
er treatment showed positive values on PCI, and the other treatments showed negative values on PCI, and the major carbon sources to deter-
mine their if erences were carbohydrates, carboxylic arids and amino acids. The il invertase and urease activities also appeared the high-
est in bio-compund fer ilie tem, reaching 7 74 glucose(24 h and I I5 mg NH Ng.(3 respectively At the same
nutient rates, applieation of the io-ompund ferilizer could signicantly improve the functional diversities of soil microbe communities
and increase the soil enzyme activities
Keywords: bio-compound fertilizer; functional diversity; soil microbial community soil enzyme activity
收稿日期:2011-10-31
基金项目:国家自然科学基金(40871119,41171203);农业部“948"项目(2010-Z20)
作者简介:邵丽(1985一),女,受徽淮北人、在读硕士,主要从事微生物肥料研究。E-mail:aoli1723@126eom
*通讯作者:谷洁E- mail: gujoyeresina, com
万方数据