库页红景天多糖的抗氧化作用评价.pdf

南方农业学报 JQURNAL OF SOUTHERN ACRICULTURE2013,44(3):493-496
ISSN 2095-119]: CODEN ANXA1R
http://www.nfnvxh.com
DOI:10.39%69:is.2095-1191.2013.3.493
库页红景天多糖的抗氧化作用评价
郭辉娟
(河北大学生命科学学院,河北保定071002)
摘要:【旧的〕探讨库页红景天多糖的体外抗氧化作用,为红景天多糖的合理开发利用提供科学依据。【方法]采用
荑嘌昤一黄嘌呤氧化酶鲁米诺发光体系、抗坏血酸-CuSO-酵母-HO2系统及荧光法分別测定库页红景天多糖对超
氧阴离子(2)、羟自由基(?OH)、NO的清除作用,同时采用Fe2诱发脂蛋白过不饱和脂肪酸(PUFA)过氧化体系评价
库页红景天多糖的抗脂质过氧化作用。【结果]库页红景天多糖的总糖含量为42.49%,糖醛酸含量为38.28%库页红景
天多糖能有效清除、OH、NO及抑制脂质过氧化,抑制率分别达到90.50%、87.72%、70.25%和85,05%,其对应的C0
分别为3.30ug/mL、0.90 mg/ml、20.00 Hgml和0.90mgmL。[结论]库页红景天多糖是一种良好的天然抗氧化剂,可
应用于食品、医疗和保健等方面
关键词:库页红景天;多糖;抗氧化作用;超氧阴离子;羟自由基;NO;ICs
中图分类号:Q539;R2855
文献标志码:A
文章编号:2095-1191(2013)03-0493-04
Antioxidation of polysaccharide from Rhodiola sachalinensis
GUO Hui-juan
(College of Life Sciences, Hebei University, Baoding, Hebei 071002, China
Abstract: [Objective This research aimed to investigate antioxidation in vitr of Rhodiola sachalinensis polysaccha
ride( RSP), in order to provide scientific references for its reasonable utilization. [Method Xanthine-xanthine oxidase-lu
minol glow system, ascorbic acid-cus0-yeast-h20 system and fluorescence method were employed to determine the
scavenging act on of RP on superoxide anion H and NO radical. The Fe-inuced lipoprotein unsa urated fatty acid
peroxidation system were used to evaluate anti-lipid per xidation of RTSP. [Result The total and uronic acid content of RSP
was. 49% and 38.28%, respectively. RP could effectively scavenge superoxide anion, hydroxy] and nitric oxide radi
cal, and inhibit lipid peroxidation with the inhibition rates of.50%, 87.72%, 70.25% and 85.05%, respectively, and
the Icu values were 3. 30, 0.90 20.00 and 0.90 mg/ml, respectively. Conclusion RSP, an excellent natural antioxidant
(uld be: usc:d for foxed medical Ireatment and healtheare
Key words: Rhodiola sachalinensis; polysaccharide; antioxidation; superoxide anion; hydroxyl radical; NO radi
0引言
苷、红景天苷元酪醇、红景天多糖、黄嗣类、有机酸、生
〔研究意义]库页红景天( Rhodiola sachalinensis
物碱和挥发油成分等(盛长忠等,2004)。临床研究表
A.Bor)又名高山红景天,为景天科红景天属多年生明,红景天对治疗冠心病心绞痛(庆慧等,200先天
草本植物,在我国主要分布于黑龙江尚志、宁安、海林性心脏病体外循环术后炎性反应(魏福岭等,2010)和
等县及吉林长白山,其野生资源极其有限。库页红景高原地区老年慢性肺心病等均有较好的疗效(杨生岳
天为环境适应性药物,具有抗缺氧、抗輻射抗疲劳
等,2010)。天然多糖是一类重要的生物活性物质,近
抗寒冷和延缓机体衰老等功能,因此药用和保健价值年来引起人们的广泛关注( Yang and Zhang,2009),
极高(盛长忠等,2004)。【前人研究进展】目前,已从库关于红景天多糖,已有研究证实其其有降低血糖(郭
页红景天中分离出40多种化学成分,主要有红景天辉娟等,1999调节机体免疫(朴花等,2000抑制骨
收稿日期:2012-11-30
基金项目:保定市科学技术研究与发展指导计划项目(1IN032
作者筒介:郭辉娟(1975-),主要从事植物及菌根生态学研究T作,E- mail: guohuijuan2000@126com
万方数据