生物催化肉桂醇制备3-苯丙醇.pdf

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化工进展
2368
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
2010年第29卷第12期
研究开发
生物催化肉桂醇制备3-苯丙醇
张笨晦2,刘雄民1,马丽1,陈晓水
广西大学化学化工学院,广西南宁53000462广西水产研究所,广西南宁530021)
摘要:筛选到能催化肉桂醇生成3-苯丙醇的菌株CG10,该菌株被鉴定为毛簿菌( Mucor sp.)。对其进行生物
催化研究,用气相色谱-质联用仪、红外光谱仪、高效液相色仪对产物进行了表征。通过对徽生物催化反应
条件的优化及对底物的耐受性实验得到最优降解条件为:椈精质量浓度为3g几,蛋白陈质量液度为1.6g几L,反
应体系初始pH=5,肉桂醇的初始加入量为2mLL,30℃下反应24h,二次加入量为0.5mLL,反应8h,3
次加入量为0.5mILL,反应16h。在此条件下肉桂醇的转化率为99%,3-苯丙醇的选择性为92%,转化液中3
苯丙醇的产量较优化前提高了123%,达到2.9g/L
关键词:肉桂酶;3-苯丙;氢化肉桂醇;生物催化;毛霉菌
中图分类号:Q93
文献标志码:A
文章编号:1000-6613(2010)12-2368-05
Hydrogenation of cinnamyl alcohol to 3-phenylpropanol by biocatalysis
ZHANG Zuhui. 2, LIU Xiongmin, MA Li, CHEN Xiaoshui
School of Chemistry & Chemical Engineering, Guangxi University, Nanning 530004 Guangxi, China;
Guangxi Institute of Fisheries, Nanning 530021, Guangxi, China
Abstract: A CG10 strain was isolated which could transform cinnamyl alcohol to 3-phenylpropanol
The strain was proved as Mucor sp by bacteria identification. The biocatalyzed reaction product was
characterizated by GC-MS, IR and HPLC. The optimized degradation conditions were found as 30 g/L
dextrin, 16 g/ peptone with the initial cinnamyl alcohol addition amount of 2 ML/L at PH 5.0 under 30
C for 24 h of reaction. The secondary cinnamyl alcohol addition amount was 0.5 ML/L for& h of
reaction, and the third cinnamyl alcohol addition amount was 0.5 ml/L for 16 h of reaction Under the
optimized conditions, the conversion of cinnamyl alcohol was 99%, the selectivity for
3-phenylpropanol was 92%o and the yield of 3-phenylpropanol in the transformation liquid was up to
2.9 g/L, which was 123% higher than the control one
Key words: cinnamyl alcohol; 3-phenylpropanol; hydrocinnamyl alcohol; biocatalysis; Mucor sp CG10
苯丙醇又名氢化肉桂醇、利胆醇,常用于药有文献?报道在以PtC为催化剂进行肉桂醛加氢反
物及香料合成。由于其具有甜的花香香气和甜蜜钱应时,主要生成3-萃丙醇。工业上3-苯丙醇通常由
香味,我国GB276086规定为暂时允许使用的食肉桂酸乙酯催化加氢制得,收率可达85%,该法的
用香料叫。天然3-苯丙醇存在于草莓、安息香膏、不足之处是反应结束后需用大量乙醚对滤液进行提
茶叶、桂油等中,但因含量低,在国际市场上供
不应求以。姜子涛等对利用微波辅助水蒸气蒸馏收稿日期:20100-9;修改稿日期:2010315
法和传统的水蒸气蒸馏法提取的肉桂挥发油组分金项目广西科学基金项日(083200及广西科技攻关项目
进行了对比,发现前者所得3-苯丙醇含量为0.83%,
(1099061-2)资助。
作者简介:张筆晦(1979-),女,博士研究生。E-mail
高于后者0.10%。在使用催化剂对肉桂醛进行加氢hana9aon联系人:刘民,教授,博士生导师,
时,其选择性和加氢途径受催化剂的影响很大4?。研究方向为香料合成。E-mailxmliul@gxu.edu.cn