磷钨酸乙炔黑修饰电极上的阿昔洛韦电化学行为及其测定.pdf

第25卷第5期
化学研究与应用
Vol 25
2013年5月
Chemical Research and Application
May
文章编号:1004-1656(2013)05-0696-05
磷钨酸/乙炔黑修饰电极上的阿昔洛韦
电化学行为及其测定
刘琼燕,何晓英”,高渐龙,贾晶
(西华师范大学化学化工学院,四川南充637002)
摘要:本文制备了磷钨酸/乙炔黑修饰电极( PCA/AB/GCE),用循环伏安法(CV)研究了阿昔洛韦(ACV)在该
修饰电极上的循环伏安行为。结果表明在pH6.0的磷酸盐冲液中,ACV在该修饰电极上出现一明显的氧
化峰。在60~220mV?s'扫速范围内,其氧化峰电流(。)与打速平方根(uv2)星线性关系,表明该电极过程
是受扩散控制的不可逆过程。计算了电极过程的部分动力学参数:电极有效面积为0.06902cm2,转移电子数
为2,扩散系数为2.063x10°cm2:s'。运用方波溶出伏安法测定不同浓度ACV的方波溶出伏安曲线,结果
表明氧化峰电流(。)与ACV浓度在7.8x10~-1.0x10mol?'范围内呈良好线性关系(r=0.9924),检出
限(S/N=3)为3.1x10-7mol?L-',加标回收率为95.69%~107.2%。
关键词;乙炔黑;磷钨酸;阿昔洛韦;方波溶出伏安法
中图分类号:0657.
文献标志码:A
Electrocatlytic behavior and determination of aciclovir at
phosphotungstic acid/acetylene black modified electrode
IAU Qiong-yan, HE Xiao-ying",GAO Jian-long, Jla-jing
(College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, China
Abstract: A new biosensor was fabricated by the Phosphotungstic acid(PTA)and acetylene black(AB )to form a compound film on
a glassy carbon electrode(GCE) The electrochemical characterisitics of Aciclovir on PTA/AB/GCE were studied by using cyclic
voltammetry. The ACV shows the sensitive oxidation peak that in the PH 6.0 PBS buffer. The oxidation peak current was linear to
w"in the range of 60 220mvs, indicating the electrode process of ACV was controlled by diffusion. Square wave stripping
voltammetry was use to determine the concentration of ACV. The peak current of ACV is linearly change with the concentration of
ACV in the range of 7.0x10 to 1.0x10mol[ and the linear equation was/(HA)=0. 8172-17.93(10mol.L)with
the coefficient of 0.9924. The detection limit is 3. 1 mol. L(S/N=3). The spiked recoveries of the samples were95.69%
107. 2%. The PTA/AB/GCE shows the excellent selectivity, good repeatability and stability for the determination of ACV
Key words: acetylene black; posphotungstic acid aciclovir; square wave stripping voltammet
阿昔洛韦又名无环鸟嘌呤核苷( Aciclovir,用于慢性病毒性乙型肝炎、单纯性疱疹病毒、带状
ACV),是一种高效广谱抗病毒药物,临床上主要疱疹等引起的皮肤和粘膜感染。若过多服用或使
收稿日期:20130105;修回日期:2013-0301
基金项目:四川省高校科技创新团队建设项目(2010008)资助
联系人简介:何晓英(1955-),女,教授,主要从事电化学研究。F-mail:hexysct@yahoo.com.cn