As(Ⅲ)暴露对紫贻贝抗氧化酶活性和脂质过氧化的影响.pdf

第32卷第4期
海洋通报
Yol. 32 o
2013年8月
MARINE SCIENCE BULLETIN
Doi:10.11840j,issn.1001-6392.2013.04.010
Ⅲ)暴露对紫贻贝抗氧化酶活性和
肯质过氧化的影响
李巧梅12,王清,于倩12,吴惠主,由丽1.赵建民
1.中国科学院台海毕带研究所海半带环境过稈与生态修复重点实验室,山东台26403;2.中国科学院文学北京1(X019
摘要:为探讨As(m)対紫片( Kyilus grallopmrine is)胰和態乳抗氧化能!钧影啊、测
「Ⅲ)暴露30犬后、肝腺和鳃组织超氧化物技化(O)、过化嗨(T),谷胱甘肽基转移酶(CNT)的活性
化以及合晓に肽(CSH)和丙二路(MDA)含量的変化:結果发现:4s)暴对紫贝胰腺(:T、SOD.GST汗
以及ぶH含量兀显著影响,高浓度AsⅢ)暴露可诱导MIA含量的起著升高;対鰓组织前,中等剂暴露组C4T活性
以及CSH含量変对照组水半扱显著升高.高剂As(用)暴露组MDA含量较对照症落廾高:研究结果表明,低浓度
Aa()长期暴露即可导致紫贻贝疒牛氧化胁迫.子致肝胰腺和腮组独的指质过化,1As(Ⅲ)对紫蛘贝幫组的影响
较扦胰腺组更加鼠著
关键词::价呻;抗氧化酶:脂听过氧化;紫哈贝
中图分类号:5944
文献标识码
文章编号:1(U01-6932201304-0429-G5
Study on the effects of As(l)exposure on antioxidant enzymes and
lipid peroxidation of Mytilus galloprovincialis
L Qiao-mei 2, WANG Qing, YU Qian, Wt: Hui-en YOU Li-ping, WHA0 Jia-min
(1. Key laboratory of Coastal n virommental rocesses and Ecological Re di on Y tai Institute of nasal n Research
Chinese Academy a S tent s, Yantai 264003, hi 2. Uni nsity of Chin se Acalemy Scienes. Beiitg 100049, China)
Abstract: Ir. the present study, the antioxidant responses and lipid p ation in gills and digestie: lands were deter
mined to investigate subchronic toxic effects of solium arsenite on Mytilus gulloprinciadis. The mussels were tx used to
differen d ses of sodium as ite(1, 10, 1 ug/) respectively for onc month, and the enzymatic activities of glutathio
S-transferase (GT ) superoxidase islas (5OD), catalase (CAT)and I
aldehyde (MDA were measured No significant change was observed in, 5), GST activities and SH ntent of
hepatopancreas adler As( ll )( xposure; however, MDA contenl was significantl ine rease in the high dose
to the rol group As lor gill, the MDA content of high lo u inre signif antl and he T alii and GSH
content of 10 Hg As (l )group increased signif anll c mparing with thse f the cou ml grup Ihese resuls indicale
that snh-rhriaie exposure to low level of As (I )would induce oxidative stress and lipid peroxidation it h palopancrcas and
gudloprorincials, and the response of gills was more sensitive than that of hepatopancreas
Keywords: trivalent arsenic; antioxidant enzymes: lipid peroxidation: Mytilus guloprow cialis
珅(As)在自然界中普遍存在,它既有金属化物作原料的工矿企业“废”的非放,使得环境
性又有非金属性,是一种毒性较高的污染物。近年中伸污染日趋严重:日前,我国近岸海环境中砷
来、砷矿的开采和熔炼、含砷农药的使用以及用砷污染状况也较为严峻。据《中国海洋环境质量公
收稿日期:2012-11-30:修订期:2013-(1]-30
金顶日:国家际然科学基金(41206105)
作者简介:李巧杓(198)、女,硬士研究年?主要从事海洋生态毒理学研究电子邮箱:1Iy_19820514a63.com
通讯试作者:赵建民,研究员:电子邮箱: phau(@ic,d:
http://nytb.nmdis.gov.cn
万方数据