多重耐药鲍曼不动杆菌对戊二醛消毒剂的敏感性.pdf

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广东医学2015年6月第36卷第12期 Guangdong Medical Journal Jun.2015,Vol.36,No.12
专是题报道
多重耐药鲍曼不动杆菌对成二醛消毒剂的
敏感性
巫培连,越子文,陈恵玲,赵礼香,魏树全,李裕军,黄晓梅
广州医科大学附属广州市第-一人民医院呼吸科(510180)
【摘要】目的探讨鲍受不动杆茵对消毒剂的敏感性,为医院消毒措施提供参考依据。方法采用消毒剂
对细菌最低杀菌浓度(MBC)和最低抑菌浓度(MIC)的检测方法,评估鲍曼不动杆菌对3种消毒剂的敏感性。采
用PCR方法检测耐消毒剂基因qacE△1-su结果邻苯二甲醛消毒剂、纯成二醛消毒剂、2%强化成二慾消毒
剂对多重耐药鲍曼不动杆茵的MBC値分别为500、200002500mg/L,MIC值分別为250、5000315mg/L。邻苯
二甲醛消毒剂、纯成二醛消毒剂、2%强化戊二消毒剂对敏感鲍曼不动杅菌的MBC值分别为250、100001250
mgyL,MC值分别为125、2500、78mg/L邻苯二甲消毒剂、纯戊二醛消毒剂、2%强化成二醛消毒剂对质控茵
ATCC27853、ATCC25922的MBC值分别2500、1000J250mg/L,MIC值分别为125、2500、78mg/L。PCR方法
检测到7株多重耐药鲍曼不动杆菌含有耐消毒剂基因qacE△1-sul出,5株敏感鲍曼不动杆和质控藺祩未检测出
耐消毒剂碁因qucE△1-sull结论2%强化成二醛消毒剂对多重耐药鲍曼不动杆菌的MC值和MBC值較敏感鲍
曼不动杆茵、标准菌株明显升高,多重耐药鲍曼不动杄菌对消毒剂抗性的产生与耐消毒剂基因qaeF△l-sul有关。
关键词】消毒剂;鲍曼不动杄茵,最低杀菌浓度;最低抑茵浓度;耐消毒剂基因
Study on susceptibility of Acetobacter amnii to common disinfectants. W Pei-lin, ZHAO Zi-wen
CHEN Hui-ling, ZHAO hu-xiang, WE Shu-quan, LI Yu un, WANG Xi-mei. Department of Respiration, First
Peoples Hospital ofguangzhou Atfiliated to Guang Medical University, Guanghou 51080, China
Corresponding author: ZLAO Zi-wen. E-mail: hoz@ yeah. ne
Abstract] Objective To study the susceptibility of Acinetobacter baumannii Lo common disinfectants and to pro
vide more practical measures for hospital disinfection. Methods The susceptibilities of multidrug-resistant Acinetobacter
baumannii to three common disinfectants in hospital were evaluated by determination of minimum bactericidal concentration
(MB)and minimum inhibitory concentration(MIC). Disinfectant resistance gene qaceal-sull was detected by PCR
Results The MBCS of formaldeyde infectant, glutaraldehyde disinfectant and 2% strengthening glutaraldehyde disin
fectatht against multidrug-resistant Acinetobacter baumannii were 500, 20000 and 2 500 mg/L, respectively, while the
MICS were 250, 5 000 and 315 mg/L, respeetively. The MBCS of formaldehyde disinfectant, glutaraldehyde disineclan
and 2%o strengthening glutaraldehyde disinfectant against sensitive Acinetobacter baumannii and standard strains were 250
ly, while the MICS were 125, 2 500 and 78 mg/1, respectively. The qacea1-sul
gene was detected in 7 multiple drug resistant Acinetobacter bauman i straits, while not in 5 strains of sensitive strains of
Meinetobucter baumannii and quality controls. Conclusion The MBC and the MIC of multidrug-resistant Acinetobacter
baumannii to disinfectants are significantly increased. Multiple drug resistant Acinetobacter baumanni resistance to disin
fectant is associated with disinfectant resistance genes qaceal-sull
Key words] disinfectants; Acinetobacter baumannii; MBC; MIC:; disinfectant resistance genes
鲍曼不动杆菌( Acinetobacter baumannii)是一种具镜,污染严重,清洗消毒难度大,不严格消毒导致的院
有获得耐药性和克隆传播能力的革兰阴性菌,多重耐内感染常有发生,内镜的清洗消毒问题是值得关注
药鲍曼不动杆菌日益增多。多重耐药鲍曼不动杆的。滥用抗生素是导致多重耐药鲍曼不动杆菌流行
菌、泛耐药鲍曼不动杆菌引起的感染已是重症监护病的重要原因,但对常用消毒剂对其影响报道不多,我们
房(ICU)医生面临的棘手难题-3。1CU频繁使用内分析CU环境中多重耐药鲍曼不动杅菌的流行与消毒
剂的耐药性有关,ICU常用的内镜消毒剂是2%强化戊
广¨东省自然科学基金资助项目(编号:101510000100005)
二醛,为此我们进行本研究,旨在了解多重耐药鲍曼不
州市医药卫生科技项目(编号:201102A212019,20151AO11004
动杆菌对2%强化戊二醛消毒剂的感性,寻找暴发
▲在读硕土研究生
院内感染的原因
△通信作者。E-mail: zhaozw@ycah.ncl