苦参碱对淋巴瘤Raji细胞的增殖及survivin蛋白表达的影响.pdf

现代肿瘤医学2010年09月第18卷第00期
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应答。④分离纯化患者自身肿瘤细胞来源的 exosomes,不仅
linical phase LII Trial J]. Clin Cancer Res, 2005, 11(11)
包含已知和未知的几乎全部肿瘤抗原,而且其肿瘤抗原/
4160-4167.
MHC-Ⅰ复合物非常适合临床应用。因此,值得深人研究其[3]ト宁,孙秉中,李奇灵,等.K562细胞分泌的 exosomes的免疫电
生物学特性,有望为恶性肿瘤免疫治疗开辟出新的途径
镜研究J].第四军医大学学报,2005,26(5):241-243
基于 exosomes的大小及密度,本实验采用一种新的快速
4] Strome SE, Voss S, Wilcox R, et al. Strategies for antigen loading of
超滤的方法来纯化小量 exosomes,该方法较传统的方法提高
dendritic cells to enhance the antitumor immune response [J
Cancer Res,2002,62(6):1884-1889
了产量及纯度,因而有可能用于临床试验リ。结合 exosomes
的理化特性,受病毒学免疫电镜研究的启发,突破国外超低( s Yamanaka R, Yajma N,AbeT,eal. Dendritic cely- ased glio-
ma immunotherapy[ J]. Int J Oncol, 2003, (1): 5-15
温组织切片术及免疫标记技术的应用局限,本实验采用[6] Fecci PF, chell DA,AacE,ata. The histo,ea
改良的固相免疫电镜法,对 exosomes表面表达的免疫分子进
and clinical use of dendritic cell-based immunization strategies
行观察。该法标本用量少,简便快速,省力,直观可靠,敏感
in the therapy of brain tumors [J]. J Neurooncol, 2003, 64(1-
性高,可供快速观察分离得到的 exosomes,并写其他亚细胞
2):161-167
器进行鉴别,容易推广,较前者有一定的优越性。经透射电[7 Cheever MIA, Disis ML, Bemhard H,etal. Immunity to oncogenic
镜观察了胶质瘤细胞释放的 exosomes,并通过免疫电镜方法
proteins [ J]. Immunol Rev, 1995, 145: 33
证明了 exosomes表达胶质瘤抗原MACE-1,细胞间粘附分【8] Rosenberg SA. Cancer vaccines based on the identification of genes
子ICAM-1。MAGE-1为胶质瘤特异性抗原,ICAM-1是
encoding cancer regression antigens [J]. Immunol Today, 1997, 18
种粘附分子,参与细胞靶向。这些分子在 exosomes中发挥
(4):175-1
着呈递抗原到抗原提呈细胞,引起特异性免疫反应的作用。
9 Cheever MA, Chen W. Therapy with cultured T cells: principles re-
ed J. Immunol Rev, 1997, 157: 177-1
本研究发现外周血DC可负報)作递胶质瘤细胞米源的[10、x、Suリ tumors to escape immune recognition: immuno-
wakami
exosomes,激活自体T淋巴细胞,诱导出具有肿瘤特异性的
CTIs,与胶质瘤胞共问培养后显示明显的抗肿瘤效应。
cnetherapy and the cell biology of major histocompatibility com
exosomes致敏的DC激活CTls的能力显著高于肿瘤冻融抗
plex class I [J]. J Immunother, 1993, 14(3): 182-190
原致敏的DC组。结合其他学者的研究结果,我们认为胶质[L11] Morse MA, Garst], Khan S,eta. Preliminary results of a phase Lレ
瘤瘤细胞来源的 exosomes负载外周血DC可有效抑制胶质
I study of active immunotherapy with autologous exosomes load
瘤细胞的生长,具有较强的体外杀瘤活性,因此有望作为
ed with MAGE peptides in HI A A2 patients with stage II/IV
种有效的临床洽疗策略和手段。
non-small cell lung cancer J]. Proc Am Soc Oncol, 2002, 2
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(编校:张志明
苦参碱对淋巴瘤Rj细胞的增殖及 survivin蛋白表达的影响
唐友红',钟美佐',余兴2,刘薨',黄进
Effect of matrine on the proliferation of lymphoma Raji cells and survivin expression
TANG You-hong, ZHONG Mei-zuo, YU Xing, LIU Wei, HUANG Jin
Department of ncology, Xiangya Hospital of Central South University, Hunan Changsha 410008, China
Abstract] Objective: To investigate the growth inhibiting effects and the possible mechanism of matrine on Raji
cells Methods: Human Burkitt lymphoma cell Raji was co-cultured with matrine