肉豆蔻挥发油体外抗病原微生物活性研究.pdf

成都医学院学报2014年第9卷第6期 Journal of Chengdu Medical College,2014,Vo.-9,No.6
675·
doi:10.3969/j.isn.1674-2257.2014.06.006
?论著?
肉豆蔻挥发油体外抗病原微生物活性研究
胡杰,代婿,王城城,陈玉平,唐婉林,代敏≌,万莉
成都医学院检验医学院(成都610500)
摘要】目的探讨肉豆蔻挥发油体外抗病原微生物活性。方法采用琼脂平板2倍稀释法测定肉豆蔻挥
发油对大肠埃希菌和金黄色葡萄球菌等16种67株临床分离菌和白偎丝酵母菌14株、非白假丝酵母菌1株临床分
离真菌的最低抑菌浓度(MIC);单因素方差分析法比较肉豆蔻挥发油对金黄色葡萄球菌、白假丝酵母菌、大肠埃希
菌、洛菲不动杆菌与肺炎克雷伯菌等不同种属真菌和细菌的抑菌活性。结果肉豆蔻挥发油除对铜绿假单胞菌抑
茵活性相对较低(MIC为4.25~8.50mg/ml.)外,对其余受试细菌有较强的抑菌活性(MIC为0.07~1.06mg/
mL);肉豆寇抨发油对白假丝酵母菌和非白假丝酵母菌均有较强的抗真菌活性,MIC分别为0.07~0.53mg/ml.
和0.07mg/mL。统计分析表明,肉豆蔻挥发油对白假丝酵母菌的MIC明显低于对大肠埃希菌和金黄色葡萄球菌
的MIC(P<0,01),对肺炎克雷伯菌的抑菌活性明显强于对金黄色葡萄球菌、大肠埃希菌的抑菌活性(P<0.01,
P<0.05),对其它种属细菌之间的抑菌活性差异无统计学意义(Pン0.05)。结论肉豆蔻挥发油具有较强的抗细
菌和抗真菌活性,可用于新型抗菌药物的开发,运用前景广闲
【关键词】肉豆蔻;挥发油;抗细菌活性
中图分类号】R285.5【文献标志码】A
The Research of Vitro Antimicrobial Activity of the Essential Oil from Myristica fragrans Houtt Seeds HU ie, DA
J uan, WANG Cheng-cheng, CHEN Yu-bing, TANG Wan-lin, DAI Min, WAN Li School of Laboratory
Medicine, Chengdu Medical College, Chengdu 610500, China
[Abstract] Objective To investigate the antimicrobial activity of the essential oil from nutmeg, lay the
foundations for the development and application of Myristica fragrans Houtt in treating infectious diseases. Methods
The minimum inhibitory concentrations(MICS) of the essential oil of the seeds from 65 strains of bacteria, 14
strains of Candida albicans and 1 strain of nox- Candida albicans, like E coli and S. aureus, were determined by
agar double dilution method. The datum were analyzed by the statistical software SPSS 17. 0. And then the
multifactor analysis of variance (ANOVA) was used to compare the differences among the different microorganisms
Results 1) The essential oil from Myristica fragrans Houtt seeds had relatively low activities on Pseudomonas
aeru ginosa, with MCS ranging from 4. 25 mg/mi, to 8.50 mg/mi, and except for that, the essential oil had strong
antibacterial activities against other experimental bacteria, with MICS ranging from 0.0 mg/ml to 1.06 mg/ml; 2)
The nutmeg oil had strong activity against Candida albicans and nom Candida albicans, with MICS 0.07-0.53 mg/
MI. 0. 07 mg/ml, respectively. As the datum shown, the nutmeg oil had lower antimicrobial activity against
Candida albicans than that of S. aureus and E. coli(P<0. 01), and the nutmeg oil had higher antibacterial activity
against Klebsiella pneumoniae than that of S. aureus and E. coli(P<0.01). Conclusion The essential oil from
nutmeg showed broad-spectrum antimicrobial activity. In consequence, it can be a potential candidate as
treat the infectious dis
[Key words] Nutmeg; Essential oil; Antibacterial activity
基金项目:国家级大学生创新创业训练项目(NO):201413705009);成都医学院大学生创新性实验项目(NO:CX201209);四川省卫生厅项目
NO):130307);成都医学院校基金(NO:CY711-002),成都医学院校基金(N(O):10Z10-022)
△通信作者:代敏,E-mail:daimin1015@163.com