大分子拥挤环境中脂肪酸影响磷脂囊泡相变的差示扫描量热研究.pdf

物理化学学报( Wuli Huaxue Xuebo
August
Acta Phys. -Chim. Sin. 2016, 32(8), 2027-2038
2027
Article
doi:10.3866/PKU.WHXB201605033
www.whxb.pk
大分子拥挤环境中脂肪酸影响磷脂囊泡相变的差示扫描量撚究
王娇杨利军”朱甜甜汪慎之陈忠秀
(浙江工商大学食品与生物工程学院,杭州310018
摘要:脂防诱导的碘脂膜的热力学行为对于认识细胞内复杂的机制有着重要意入的人在研冗、酸
磷脂膜相互作用吋大都在稀溶液中进行;拥挤环境下脂肪酸诱导僯脂煐的椤
道。本文以二?
豆蔻酰磷脂酰胆碱(DMPC)构建襄泡模型,采用差示扫描量热法系统地研
浓度
分子量的聚
乙二醇(PEG)拥挤环境中不同结构的脂肪酸对DMPC磷脂囊泡相变的影响。
表明,在拥挤环境中,
PEG对纯的磷脂囊泡相变的影昫与大分子的分子量和浓度相关。对于脂
A/DMPC),PEG的
存在对甕泡相变产生显著影响。在所考察的分子量和浓度范围内,PE使 FAR/MPC心相变增加。短链饱
和脂肪酸、不饱和脂肪酸原本使DPMC囊泡相变降低,但P缩小
度
导致相变增加。进
步的研究表明,在大多数情况下,PEG对FA/DMPC的相变具
增驻
响均与大分子的分子
量和浓度相关。另外,随着PEG浓度的升高,磷脂鞭泡的协同单
渐降低,表明拥挤环境会影响磷脂
双分子层的均一性,使协同发生相变的分子数降低。本文的
拥挤环境能够对扰动的磷脂双
分子层起到一定的修复作用,这一现象在生物農相域不司
关键词:大分子拥挤:礫脂囊泡;脂肪酸:相变:示扫描
中图分类号:O648
Phase Transition of Phospolh Ve/ic, s Induced by Fatty Acids ir
Macromolecular Crowding: a ifferenti-/Scanning Calorimetry Study
WANG Jiao YANT
ZHU thh-tian WANG Shen-zhi CHEN Zhong-xiu
(College of Food Biology Engine
gongshang University, Hangzhou 310018, Zhejiang Province, P R. China
Abstract: Investiga
the therme namics of fatty acid(FA modulation of lipid membrane behavior is
important to unders d th mechanisms that occur in cells. Previous research of the interaction between FAS
and lipid membra has n per d in iue lution an n study has ocused on the effect of an extemal
crowding medium on the
ansition of the lipid membrane induced by FAS. In this paper, the effect of
va ous mo cular weghts cncenaon of polyeth en glycol(PEG)on the phase transition of 1. 2
dimyristoy-glycero-3-phi sphocholine(DMPC)vesicles mixed with FA was systematically investigated by
anning calorimetry(DS). The results show that the effect of PEG on the phase transition of pure
DMP/vesi
bimolecular weight and concentration dependent. The presence of PEG significantly
hanes the has ansi on of FAVDMPC vesicles Pase transition temperature()of FA/DMPC vesicles
or most of the considered concentrations and molecular weights. The original Tn of DMPC
induced by sh chain saturated FA or unsaturated FA increased in the presence of PEG. Further investigation
revealed that in most cases a collaborative effect of molecular crowding existed and the effect of PEG on Tm
Receiv Feby My 29, 2016; Revised: May 3, 2016; Published on Web: May 3, 2016
Corres
authors.CHENZhong-xiu,Email:zhxchen(@mail.zjgsu.edu.cn:Tel:+86-11-28008980
YANG
mailspxy21019@126.com
The project was supported by the Zhejiang Provincial Top Key Discipline of Food Science and Biotechnology for Financial Support,
China (JYTSP20141012
浙江省食品科学与生物技术“重中之重”基金( UYTSP20141012)资助顼目
C Editorial office of Acta Physico-chimica Sini