富GABA米糠改善大鼠氧化应激和肝功能损伤.pdf

2017年4月
食品研究与开发
160
第38卷第7期
Food Research And Development
营养保健
DOI:10.3969/jsn,1005-6521.2017.07.035
富GABA米糠改善大鼠氧化应激和肝功能损伤
周中凯,刘志伦,张惠媛
天津科技大学食品工程与生物技术学院,天津300457)
摘要:研究富伽马魚基丁酸(γ- aminobutyric acid,GABA)米糠有效政善高脂饮食诱发大鼠氧化应激和肝功能损伤。
添加GABA前体物I-谷氮酸,通过原米糠中脱酸酶转化获得富GABA米,其中GABA的含量由32mg/100g升高至
242ng100g原米、GABA米糠分别添加到高脂饲料,试验大鼠分为正常对照组、高脂饲料组、普通米糠组和富
GABA米糠组,考察普通米糠、富GABA米槺对高脂饮食大鼠的体重、肝指数、肝功能及氧化应激的影响。富CABA米檍
组大鼠体重明显低于高脂饲料组大鼠和普通米糠组大鼠(p<0.5),富CABA米糠在减轻肝脏细胞脂肪变性、降低转酶
及碱性磷酸酶、改善肝脏氧化应激等方面优于菁通米糠组大鼠
关键词:普通米糠;富GABA米糠;高脂饮食;肝功能;氧化应激
The Improvement in Oxidative Stress and iver Function Damage in Rats by Gaba-enriched Rice Bran
ZHOし Zhong-kai, LIL Zhi-lmn,∠ HANG Hui-yuan
( College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin
300457, China)
Abstract High-fat diet induced oxidative stress and liver function injury in rats were effectively improved
through investigating Gaba-enrich rice bran. The natural rice bran was transformed into the Gaba-enrich rice
bran by virtue of aminobutyric acid enzyme, which was acquired by adding GABA precursor substance L-GLU
tamic acid into it. It was clearly found that the content of GABA increased from 32 mg/100 g to 242 mg/100 g
Firstly, the natural rice bran and Gaba-enrich rice bran were added into the high [at feedstuff respectively
Then. the effects of Gaba-enrich rice bran on high-fat diet rats weight. liver function and even oxidative stress
were studied, the experimental rats were divided into four categories-normal control group, high-fat feed
3A oup, nalural rice bran, and Gaba-enrich rice bran group,. Finally, it was revealed that the ral weight in GA-
Ba-enrich rice bran group was lower than high fat fee group rals and natural rice bran group rats (P<0.5)
Therefore, it was proved that the Gaba-enrich rice was superior to the natural rice bran in reducing li
steatosis cells, aminotransferase, alkaline phosphatase and in improving liver oxidative stress
Key words: nalural rice bran Gaba-enrich rice ran; high fal diel; liver function; oxidative slress
随着人们的生活水平的不断提高,与之伴随的高物都过于昂贵或副作用大,因此通过食物干预有效控
脂饮食成为了普遍现象,由此引发的肥胖、高血压、代制体重与改善肝班功能成为研究热点之
谢综合症、非酒脂肪肝等疾病也越来越多,威胁人
米糠做为稻米生产的副产物,含有低致敏性蛋
们的身体健康。特別是肥胖,不仅增加心血管疾病、白、纤维素、谷维素、神经酰胺、二十八烷醇、生育三烯
糖尿病、慢性肾脏疾病等疾病患病率?,而且易引起酚等多种天然活性成分及抗氧化物质。伽马氨基丁
脂肪肝,引发肝脏脂肪代谢紊乱及造成肝功能受损,酸(γ- aminobutyric acid,CABA)是一种从细菌到植物
降低体重是保持肝脏健康的重要策略之一,而传统药和脊椎动物普遍存在的非蛋白质氮基酸。它可以由
基金项目:科技部农业科技成果转化(2014CB2A100527):国家星火
L-谷氨酸经谷氨酸脱羧嗨催化转化阿米,是一种重要
计划项日(2015cA610003)
的抑制性神经递质。同时也貝有降低血脂的功能,及
作者简介:周中凯(1964-).男(汉),教投,博上,研究方向:谷物科改善氧化应激、改善肝肾功能的作用,而在普通米糠
学与营养
中GABA含量较低,其功能性表达较弱,为此本试验
万方数据