一个新的Br蛋白基因部分序列测定及分析 Analysis of Partial Sequence.pdf

遗传 HEREDITAS( Beijing)26(3):343~348,2004
研究报告
一个新的Br蛋白基因部分序列测定及分析
许学伟,吴敏,阮红,黄伟达2,迪丽拜尔?托乎提
(1.浙江大学生命科学学院,杭州310027;2.复且人学生命科学学院,上海200433:3.新疆师范人学生物系,马鲁木齐830054
摘要:从新疆北部艾比湖分离纯化到极端嗜盐古生菌AB1,采用PCR方法扩增了其16 SRRNA基因(16 S FDNA)
和编码螺旋C至螺旋G的细菌视紫红质( bacteriorhodopsin,Br)蛋白基因片段,并测定了基囚的核作酸序刎。基丁
16 S TDNA序列的同源性比较及系统发育学研究表明,AB1是 Natromococcus属中新成员。通过对菌株ABL的Br
貨白亲水性分析表期,AB1的Bxr貨白与已报道Br貨白有类似的超二級结构,进?步的策白质序列滎合比树结果
表明,AB1中Br貨白螺旋C至螺旋G的氮基酸序列与其他菌株差异明。研究结果表明菌株AB1的Br貨白是
种新的Br蚩白。
关键词:嗜盘古生菌:细菌视紫红质;16 SRDNA
中图分类马:Q517,Q78
义献标识码
文章编马:0253-9772(2004)03-0343-06
Analysis of Partial Sequence of the Gene for A Novel Br Protein
XU Xue-wei, WU Min, RUAN Hong, HUANG Wei-da, Dilbar Tohty'
(1. College of Life Sciences. Zhejiang University, Hung hou 310027. China;
2. College of ife Sciences, Fudan University, Shanghai 200133, China:
3.De of Biology, Xinjiang Normal University, Urumgi 830054, China
Abstract: strain of halophilic archaeum ABL was isolated and purified from Aibi Lake located in the north of Xin
jiang Uygur Autonomous Rcgion. Partial DNA fragment cncoding a batcriorhodopsin(Br) protcin as well as 16s
RRNA of ABI was amplified by PCR, and their nucleotide sequences were determined subsequently. On the basis of
homology and phylognetic analysis about 16S RRNA gene (16S RDNA), it could be speculated that the strain ABL is
a novel member of the genus Naironococcu. The hydropathy analysis of Br fragment revealed that the ABL Br had
a transmembrane heptahelical structure similar to that of other Brs. On the other hand, homology alignment using
the deduced partial amino acid sequence of Br protein of ABL with other Br proteins showed that ABL Br protein is
obviously different to others. These facts indicated that the Br in halophilic archaeum ABL is a new Br protein
Key words: Halophilic archaea; bacteriorhodopsin; I6S TDNA
细菌视紫红质( bacteriorhodopsin,Br)是嶝Br和人工改造的Br蛋白用于研究Br结构和功能,
古生菌紫膜上的性一自质,并以7次を螺旋(A-并结合品体学研究,的明其光反应和质子泵机制,
G)跆膜定位丁其质膜上.。从 Halobacterium成为目前Br貨自研究中的一个热点2-。
halobium分离的Br蛋白是出248个氨基酸组成,
我们在新疆北部艾比湖中分离纯化到耐盐范围
分子质量约为26kDa,第216位的赖氨酸通过希夫在20%~25%的嗜盐古牛菌AB1,基」16 S RRNA
碱基和牛色闭视黄醛分子相连。将野外分离的基因(16 S RDNA)形列进行了系统发育分析,随后
收稿凵期:2003-06-24;修回凵期:2003-12-02
基金项日:国家白然科学基金资勘(30370029)项日 Supported by National Natural Science Foundation of China(30370029)
作者简介:许学伟(1978-),男,仞士,研究方向:嚐盐古生菌系统分类及进化。 E mail: XXW]l(@ hot mail.cor
通讯作者:吴敏(1958-),男,教授,研究方向:分子遗传。E-mail:wumin@cls.ziu.ecdu.cn