磺胺嘧啶分子印迹电化学传感器的制备及其快速检测食品中磺胺嘧啶药物残留.pdf

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时间:2018-01-12
品科学
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磺胺嘧啶分子印迹电化学传感器的制备
及其快速检测食品中磺胺嘧啶芶物残留
玲钰,高林,高文惠
(河北科技大学生物科学与T.科学院,河北省发轉工科技术研究中心,河北石家H050000
摘要:以胺啶为模板分了,邻氨基苯岭为功能単体,采用紫外光普法优化二者比例,以羧基化多蝰碳
纳米管和纳米金为修饰材料,用滴涂法修饰歧碳电柲,在高氯酸-高氯酸钠溶液(pH=5.5)中电擬合形成擬
部氨基本盼膜,制各了磺胲喀啶分子印迹电化学传感器。实验选用含0.5 mol/L KCI及5 Iimol/L
KFe(CN)sK4Fe(CN)6l的水溶液为表征浴液,采用循环伏交法和差分脉冲伏交法研究了传感器的电化学响
应特性,优化了制铬条件,探究了印迹传感器对板分子及其结构类似物的选抒性响应忭能,并将该传感
器应用于食品中磺胺啶药物残留的快速检测。在最佳条件下,胺啶浓度在1.0×108molL~2.0x106
mol.范园内线性关系良好,檢出限为3.3x10mol,样品加标半判山收率在83.50%~97.80%之间,相対
标淮偏差<4.06。该传感器制作成本低,操作简使快速,检测火鲰度高,抗干扰能力強,稳定性?,具有
良好的成用前景
关键词:磺胺啼啶;电极修饰材料;分子印迹聚合物;电化学传感器;紫外光谱法
Preparation of Sulfadiazine Molccularly Imprinting Elcctrochcmical Sensor and Rapid Dctcction
f Sulfadiazine Residues in Foods
/HO Lingyu (AO Lin. AO Wenhui*
(College of Bioscience and Bioengineering, Center for Termentation Engineering of Ilebei Province
Hebei University of Science and Technology, Shijiahuang 050000, China)
Abstract: Using sulfadiazine as Template molecule, o-aminophenol as unctional monomer, the ratio between the
two was optimized by UV spectroscopy. The glassy carbon electrode was modified by dripping method with
carbonylated multi-walled carbon nanotubes and nano-gold so, and poly (o-aminophenol)membrane was lormed
by electropolymerization in perchloric acid-sodium perchlorate solution (PH= 5.5 ), sulfadiazine molecularly
imprinted electrochemical sensor was prepared suceessfully. In the experimenl, the aqucous solution containing 0.5
MOLL KCI and 5 mmoll[e(CN) used as characterization solution, the response characteristics of the
electrochemical sensor were researched, the preparation conditions of the sensor were optimized, the selective
收稿H期:2017-10-10
基金项:河北省科技计划项目(16275503D)
作者简介:赵玲钰(1
女,硕:研究生,研究方向为食品安全与检测。F-mai:2602516149Gq
通信作者:南文惠(1963),女,教授,博士,研究方向为食品安全与质量控制技术。E-mail
wenhuigao(@12
时间:2018-01-12
品科学
网络预发表
response of the sensor to the template molecule and ils analogues was investigated by cyelic voltammetry and
differential pulse volt ametry, and the sensor was applied to the rapid detection of sulfadiazine residues in foods
Ihe results showed that under the optimum conditions, the linear range was good at the concentration of 1.0x10-8
I. 0106 mol L the detcetion limit was 3.3 x 10 moll. the recoveries were betwcen 8350 and
97.80 %, and the relative standard deviations were less than or equal to 4.0 % The sensor has low cost, easy and
rapid operation, high sensitivity, strong anli-intcrfcrencc ability, good stability and good application prospect
Cey words: sulfadiazine: electrode modification material: molecularly imprinted polymer; electrochemical sensor:
UV spectroscopy
中图分类号:O631.3文献标志码
黄胶类药物是一类应用较普遍、效果良好的抗菌药物,主要対革一氏阳性菌及一部分革一氏阴性
菌有较弘的抑制作用,曾被广泛用于人类肠道和呼收道疾病的治疗。长期以米,横胺类药物在帝牧养
烛业中起倒较人作用,然而牧养嬗业者对胺类药物的不科学使用及滥用造成些动物源性食品中
磺胶类药物的严重超标。经调查,畜禽养殖业中猪、牛及鸡的磺胺类药物使用量最大。因此,为保
人体健,对该类禽肉中胺类药物残留的监管及检测不容忽视。我国对磁胲类其增效剂的使
用有比较明确的规定,GB29694-2013中规定商禽?类产品磺版类药物总量不得超过10ugkg1;SNT
4057-2014中规定出口类动物源性食品中械胺类药物的测定低限为0.003mgkg,欧盟470/2009中也
明确规定,什所有动物源性食品中磺胺类药物残总量不能超过100gkg。磺胺类药物
( sulfonamides,SAs)中最共代表性,且使用暈最大、使用效某较好的足胲嘧啶( sulfadiazine,SD
其结构式如图1所示
IN
图1磺胺嘧啶结构式
Fig. 1 The Structure of sul