细菌强化修复电子废弃物污染农田土壤研究.pdf

2015.34(4):709-715
农业环境科学学报
2015年4月
Journal of Agro-environment Science
细菌强化修复电子废弃物污染农田土壤研究
陈佳亮2,刘晓文2,张晓芒3,张雅静2,方晓航2
(1.'东省环境科学研究院,州510045;2.环境保护部华南环境科学研究所,广州510655;:3.'东省环境保护宣传教育中
州510630)
摘要:通过富集、驯化、分离,从清远市电子废弃物拆解区污染上援中得到四种耐性菌株,经菌落形态、扫描电镜分析以及16S
DNA技术鉴定得出菌株HS-01、JH-02、YB-03、JY-04分別为海水芽孢八香球菌( Sporosarcina aq uimarina)、佐吕间湖生芽孢八叠球
菌( Sporosarcina saromensis)大芽孢杆菌( Bacillus me gcterium)、甲基营养型芬孢杆菌( Bacillus me thylotrophicus)2选取非矿山型东
南景天( Sedum alfredi)为实验植物,在温室盆栽实验条件下,强化修复模拟的Cd、Cu、P污染土填。结果屁示添加细菌JH-02的东
南景天地上部重金属提取量相对最高,JY-04次之,均显著高」其他各组(Pk0.05):重金属形态分析显示细菌JH-02与JY-04对上
重金属的活化作用相对最好,可交换态含量明显高」其他两种细菌组与末加菌组(P<0.05)。实验结果表明,JH-02与JY-04这两
种细菌可能通过代谢活动产生的分泌物溶解了上壤中重金属,最终促进了东南景天的提取以及根部重金属向茎叶屮转移,可达到
强化修复的效果,具有一定的应用前景。
关键词:电子废奔物;重金属:耐受细菌;农田土壤;强化修复
中图分类号:X53文献标志码:A文章编号:1672-2043(2015)04-0709-(07doi:10.11654/jae.2015.0)4.015
Bacteria-enhanced Remediatio of Electronic-waste Polluted Farmland Soil
CHI'N Jia-liang.lu Xiao-wen,/HA NG, Xiao-man.H A G Ya-jing, FAN Xiao-hang
(1. Guangong Prvine al Academy of Environmenla Seine Guang hou 510045, China 2. out China Inslilulc of Fnvironmenlal Sciene
MEP, Guangzhou 510655, China; 3. Centre of Environment Protection Propagation and Eucation of Guangdong Guangzhou 510630. China
Abstract: Qinguan is one of the biggest electronic waste(e-waste ) dismantling areas in China. Over 20-year operations of individual e
waste workshops there have resulted in severe pollution of the surrounding soils by Cd, u and P. Remediation of e-waste polluted soils has
drawn seicnlilie and public allenlions. In our previous sludy, our slrains of mcla toleran bacleria were identified rom the soils conlaminal
ed by e-wastes in Qingyuan. using colony morphology analysis, scanning electron microscope and 16S RDNA technology. They were
Sporosarcina uim(hs-1 Spos mensis(J- 2). Bill megrim(yb-03 )and Bacillus meth lotrophcus( JY
4 ) Here we used these four strains and a non-contaminated ecotype of plant Sedum alfredii to test their potential for remediating soils pol
luled by Cd, Cu and P under greenhouse conditions. Resulls show Ihal Ihe shots of S. al/redi accumulaled Ihe highest amounl of hea
melas in Ie presence OFJH-02 bacteria. ollowed by JY-04 bacleria. ac a JH-2 and JY-04 xii r performane in mobili
ing heavy metals, and exchangeable metal fraction was significantly higher in the JH-02 and Y-04 treatments than in other bacterial treat
ments(F<. 05 ) Our present study suggest that the bacteria. 11-02 and J Y-04 may be able to enhance the extraction of soil heavy metals by
S. alfredii and thus have a potential to be applied to the field
Keywords: cleelronic waste; heavy meals; melal-loleranl bacleria; farmland soil; enhanced remediation
电子废弃物足指各类报废的家用电器与办公电方面,它是可再生利用的重要资源,可回收如铜、铝、
子户品,如电脑、复印机、冰箱、电视机、移动电语等,铅、锌、铁、金、银、铂等贵重金属,因开发成本远低于
是由塑料、金属和其他材料制成的复杂混合体习
矿山一次资源,电子废弃物又被人们称为“城市矿
l”;另一方面,它又是污染物,其中包含的铅、镉、求
收稿日期:2014-11-18
铬、钡、铍、多溴联苯、多溴联苯醚等均对人类和牛态
基金项目:中央级公益性科研院所基本科研业务专项(206030201-21)
者简介:陈佳亮(1987ー),男,甘张掖人,硕十,主要从事重金属
系统有害,如果处理不当,将会严重污染拆解区周边
染生物修复技术研究。E-mail:chenjialiangemail@163.com
环境
通信作者:方晓航E-mail: fangxiaohang(@scics.og
清远市是中国最大的电子废弃物拆解基地之一,