瞬时感受器电位离子通道4在大鼠背根神经节持续受压后异位放电中的作用.pdf

中圆复区霄怎2013年,第28卷,第3期
基础研完
瞬时感受器电位离子通道4在大鼠背根神经节
持续受压后异位放电中的作用
范真真曲玉娟魏慧王永慧1马剑锋岳寿伟3
摘
目的:利用在体神经纤维电生理技术记录大鼠背根神经节(①RC持续受压(CD)后的异位放电情况,明确瞬时感受器
电位离子通道4TRPV4)是否参与了CCD后受损DRG的异位放电。
方法:共采用35只 Wistar大鼠。制备大鼠DRC的CD模型,分别于术前和术后测量损伤侧的机械痛阈和热辐射刺
激缩爪反应潜伏期。利用在体神经纤维电生理技术分别记录正常组大鼠DRG及CD组、CCD+钉红(RR)组、CCD
佛波醇(4α-PDD)组受损DRC神经元的异位放电情况。
结果:持续压迫明显降低大鼠损伤侧的机械痛阈和热辐射刺激缩爪反应潜伏期(n=30,P<005);CCD组可以记录到
受损DRG神经元的异位放电,放电率约为67%,而正常组DRG的异位放电率约为4.5%;以RP家族阻断剂钉红
(RR100m孵育受损DRG,较CCD组受损DRG神经元异位放电的频率和波幅均明显下降(n=10,P<0.05);以
TRPV4特异性激动剂佛波醇4α-PDD)1Oμm孵育受损DRG,较CD组受损DRC异位放电频率和波幅均明显增加
(n=10,P<0.05)。
结论:CD后受损DRG可出现异位放电,TRPV4参与了CCD后受损DRG的异位放电。
关键词异位放电;瞬时感受器电位离子通道4;背根神经节;持续压迫;电生理技术
中图分类号:R338.8,R745文獻标识码:A文章编号:1001-1242(2013)-03-0193-05
The role of transient receptor potential vanilloid 4 in mediating the ectopic discharges following chronic
compression of dorsal root ganlion in ra/AN Zhenzhen, Q Yuuan EI Hi, et al//Chinese Jour
nal of Rehabilitation Medicine, 2013, 28(3): 193-197
Abstract
Objective: To investigae e role of transient receptor potential vanilloid 4(TRP 4)in mediating the ectopic di-
charges of the injured dorsal root ganglion(DRG)with chronic compression
Method: A total of 35 Wistar rats were used. Chronic compression of dorsal root ganglion(CCD) model was es-
tablished. Mechanical withrawal threshold and thermal withdrawal latency were administered before and after oper
ation. The ectopic discharges of normal DRG and injured DRG were recorded by nerve fibers electrophysiological
techniti
Result: Mechanical withdrawal threshold and thermal withdrawal latency reduced significantly after CCD(n=30
P<0.05) The ectopic discharges were present in 67% of the fibers recorded from DRG neurons injured with
chronic compression in contrast to 4.5% from uninjured DRG neurons; Compared with CCD group the freq
and amplitude of ectopic discharges decreased si nificantly after incubating the injured DRG with TRPS nhibitor
10 m RR(=10, P<0.05) Compared with CCD group frequency and amplitude of ectopic discharges increased
significantly after incubating the injured DRG with TRPV4 specific activator 10um 4PDD(=10, P<0.05)
DOL:10.3969/1issn.1001-1242.2013.03.001
*基金项目:国家自然科学基金资助项目(30872732;81071597)
山东大学齐鲁医院康复科,济南,250012;2山东大学医学院机能实验室;3通讯作者
作者简介:范真真,女,硕土研究生;收稿日期:2012-11-10
wunu, rehabi com.cn 193