大孔吸附树脂分离纯化五倍子鞣质.pdf

42012.Wo.33.NVo.20
品学
※工艺技术
大孔吸附树脂分离纯化五倍子鞣质
高燕3,李勤2,盛达成2,肖文军123,刘仲华'23,张盛2,傅冬和2
(1.湖南农业大学国家植物功能成分工程技术研究中心,湖南长沙410128
2.湖南农业大学园艺园林学院植物资源工程系,湖南长沙410128
3湖南农业大学园艺园林学院茶学系,湖南长沙410128)
摘要:以五倍子鞣质粗提物为原料,通过静态吸附与解吸实验比较NKA-2、NKA-9、HPD100、AB-8、D101、
D301、聚酰胺及732八种吸附树脂对五倍子鞣质的吸附与解吸性能,筛选得到最优树脂NKA-2。然后通过动态吸
附与解吸的单因素试验和正交试验,优化筛选NKA-2大孔吸附树脂分离纯化五倍子鞣质的工艺技术参数。结果表
明,当主要考虑鞣质得率时,最优工艺参数为上样质量浓度5mg/mL、上样流速1BVh、上样体积6BV、洗脱剂乙
醇体积分数80%、洗脱流速1BV/h、洗脱体积4BV,其鞣质得率可达85.37%,纯度可达62.99%;当主要考虑鞣质
纯度时,最优参数为上样质量浓度6mg/mL、上样流速2BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流
速3BV/h、洗脱体积4BV,其鞣质纯度可达76.97%,得率达67.78%。
关键词:五倍子;鞣质;大孔吸附树脂;分离纯化
Separation and Purification of Tannin from Galla chinensis by Macroporous Resin Adsorption
GA Yan L Qin SHENG Dachen XAOWE-LU Zhong-hua, ZHANG Sheng FU Donghe
(1. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,
Hunan Agricultural University, Changsha 410128, China 2. Department of Botanic Resource Engineering
College of Horticue and Lansce Hunan Aricl ural Universit Changsha 410128, China 3. Department of Tea Science,
College of Horticure and Landscape, Hunan Agricultural University, Changsha 410128, China)
Astra NK- maou so on esin was the best resin for the separation and purification of crude tannin
extract from Galla chinensis as demonstrated by a comparison of the effectiveness of &types of macoporous adsorption
resin in static ad sorption and desorption of tannin One-factor-at-a-time and orthogonal array design were used to
optimize dynami a o ion and e tion aames for the unication f the ue tannin extract. The optimal
process parameters for maximum tannin yield were dete mined as follow 6 BV of 5 m/ml sample was loaded onto the
column at a flow rate of 1 Bv/h and after complete adsorption, the column was then eluted with 4 BV of 80% ethanol
solution at a flow rate 1 BV/h, yielding a tannin yield of 85.37% and a tannin purity of.99%. However, the optimal
process parameters for max mum tannin rity were ete mined s fo ow 5 V o m/ml sample was loaded onto the
mn t a o te of V n afer mee n h lm a then elte wi h 4 of 80%tanl solution
at a flow rate 3 BV/h, yielding a tannin yield of 76.97%and a tannin purity of 67.78%
tannin, macrop
n; separation and purific
中图分类号:TQ425.233
文献标识码:A
文章编号:1002-6630(2012)20-0074-06
五倍子( Ga/la chinensis)主要由五倍子蚜寄生于漆树取法、超临界CO2萃取法、活性炭除去色素法以及膜分离
科植物“盐肤木”及其同属其他植物的嫩叶或叶柄后而法等。柳世萍等采用水浸提法从五倍子中提取鞣质
生成一种囊状聚生物虫瘿,具有敛肺降火、涩肠止泻
提取率在50%左右。汪咏梅等?利用活性炭除去鞣质中
收湿敛疮等多种功效们。五倍子中主要成分为五倍子鞣的色素、树胶等杂质,可使单宁质量分数提高1%左右,
质,其含量可达60%~70%2。目前,国内外对鞣质的颜色和透射率也有所改善。张太龙等创考察了料液质量分
提制技术的研究较多,如浸渍法、超声辅助法、加压提数、操作压力差、料液温度对超滤和纳滤膜分离鞣质效
收稿日期:2011-08-01
基金项目:“十ニ五”国家科技支撑计划项目(2011BAD10B00);国家农业科技成果转化资金项目(2011GB2D200010)
作者简介:高燕(1987-),女,硕士研究生,主要从事植物功能成分分离纯化研究。E-mail: gaoyan20110@ yahoo,cn
米通信作者:肖文军(1969-),男,教授,博士,主要从事植物功能成分开发利用研究。E-mlil:Xiaowenjun88@sina.com
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