肺癌患者外周血白细胞端粒DNA相对长度检测.pdf

Journal of Zhengzhou University(Medical Sciences) Jul. 2012 Yol. 47 No 4
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(2011-09-30收稿责任编辑徐春燕)
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doi:10.3969/j.issn.1671-6825.2012.04.006
肺癌患者外周血白细胞端粒DNA相对长度检测
王娜,周舫,谭善娟,王丁丁
郑州大学公共卫生学院劳动卫生学教研室郑州450001
△女,1976年4月生,硕士,讲师,研究方向:职业性肺癌,E-mail:wefngqiao(@z2u.edu.cn
关键词端粒;染色体不稳定性;肺癌
中图分类号R734.2
摘要目的:探讨外周血白细胞端粒DNA相对长度与肺癌的关系。方法:采用 SYBR Green Real Time PCR
方法检测54例肺癌患者(病例组)和77例健康对照(对照组)外周血白细胞端粒DNA的相对长度。结果:病例组
外周血白细胞端粒DNA的相对长度为(3.43t0.87),短于对照组(3.86t1.46),差异有统计学意义(t=2.093,
P=0.038)。进一步应用 logistic回归分析,把端粒长度按百分位数P3和P从短到长依次分为T1、T2、T3三层,调
整吸烟与年龄因素后,以T3层为参考水平,结果显示T2层患肺癌危险度为3.933(95%C71.139~13.580),T1层
患肺癌危险度为4.684(95%C71.377~15.936),P<0.05。结论:端粒DNA相对长度缔短可能与肺癌的发生有关。
Determination of relative telomere length in peripheral blood leukocytes
from patients with lung cancer
WANG Na, ZHOU Fang, TAN Shanjuan, WANG Dingding
Department of Occupational Health, College of Public Health, Zhengzhou University, Zhengzhou 450001
Key words telomere; chromosome instability; lung cancer
Abstract Aim: To explore the relationship between relative telomere length in peripheral blood leukocytes and lung
cancer. Methods: SYBR Green I Real Time PCR was used to measure the relative telomere length in peripheral blood leu
kocytes of two groups including 54 lung cancer patients(case group )and 77 healthy people(control group). Results: The
国家自然科学基金资助项目30972457;郑州市科技攻关基金资助项目083SGYS33261-7