酶解法提取大高良姜多糖工艺优化及抗氧化活性分析.pdf

南方农业学报 Joumal of Southem Agriculture2016,47(8):1376-1382
ISSN 2095-1191: CODEN NNXAAB
http://www.nfnyxb.cm
DO1:10.39695:is.2095-1191.2016.08.1376
酶解法提取大高良姜多糖工艺优化
及抗氧化活性分析
刘源,张孝琴,王译伟,杨刚
(西南酝科大学化学教研室,四川泸州64600)
摘要:【目的]优化酶解法提取大高良姜多糖工艺,并分析其抗氧化活性,为大高良姜多糖的有效利用提供技术支
持。【方法]以多糖提取率为评价指标,在单因素试验的基础上,采用 Plackett- Burman(PB)试验法对影响大高良姜多糖
提取率的5个因素进行筛选;根据PB试验结果,选取3个主叟影响因素,通过Box- Behnken响应面试验对提取工艺进行
优化,确定最佳艺条件;同时测定大高良姜多糖对DPPH和ABTS自由基的清除率【结粜)酶解法提取大高良姜多糖
最佳工艺条件:料液比1:24,pH6.0,酶解时间50.5min、酶解温度44℃、晦用量2%,在此条件下多糖提取率为13.53%
与传统热水浸提法比较,酶解法提取时问编短72.0%,提取率提高24.1%。大高良姜多糖对DPPH和ABTS自由基均有
校强的清除能力,其半数有效质量浓度(IC)分别为2.21mg/miL和2,15g1mL【结论]响应面试验模型能较好优化酶解
法提取大高良姜多糖工艺,优化后的T艺具有操作简单、省时高效、无毒环保等优点,提取得到的多糖有较强的抗氧
化能力,可为后续开发利用大高良姜提供技术支持。
关键词:大高良姜多糖;酶解法; Plackett- Burman试验法;Box- Behnken响应面法;抗氧化活性
中图分类号:R284.2
文献标志码:A
文章编号:2095-119(2016)08-1376
Optimization for extraction process of polysaccharide from
Alpinia galanga Willd. by enzymolysis method and its
antioxidant activity analysi
LIU Yuan, ZHANG Xiao-qin, WANG Yi-wei, YANG Gang
( Department of Chemistry, Southwest Medical University, Luzhou, Sichuan 646000, China)
[Objective I The extraction
process of polysaccharide from Alpinia galanga Willd. by enzymolysis method
was optimized in order to provide technical suppor or effectively using polysaccharide from A. galanga.[Method] With
extraction rate of polysaccharides as index, the five factors affecting extraction rate of polysaccharides from A. galanga
basis of single fa
945,g factors were selected. Then the extraction prucess was optimized byby using the Box
polysaccharide from A. galangal to DPPH free radical and ABTS free radical was determined. [Result The optimum pro
follows: solid-liquid ratio of
50.5 min, enzymolysis temperature of 44, enz me dosage of 2%. Under the above optimum conditions, the extraction
rate of polysacchande was 13.53% Compared with traditional hot-water extraction method, the enzymolysis method took
the
ased by 24.1%. Further
more, polysaccharide from A. galanga had strong ability to scavenge DPPH and ABTS free radicals, and the half effective
mass concentration(Cx)was 2. 21 mg/ml and 2.15 g/ml, respectively. I Conclusion The response surface experimental
model can opt
unite ex
traction process of polysaccharide from A. galangal, the enzymatic method can improve the extra
tion rate of polysaccharides. The extraction process has the advantages of simple operation, time saving, high efficiency
toxicity and environmental protection, ete., and extracted polysaccharide has more stronger anti
xidant activity, there
fore this process can provide scientific basis for develapment of A. galanga
Key words: Alpina galangal ill. polysacchaide enzmolysis methud; Placket-burman test; Box Beh
idant activity
0引言
姜科山姜属植物大高良姜[ Alpinia galanga((L.)Wil
【研究意义)大高良姜又名大良姜、良姜、山姜,为的根茎,是《中国药典》收录的药用植物,果实入药称
收稿日期:2016-03-10
基金项目:西南医科人学青年基金项目(20150014)
作者简介:刘源(1980-),主要从事化学工艺研究及化学教学工作,E-mil:1906370328@,c0m
方数据