猪_犬脾转移因子的理化性质及生物学特性分析.pdf

中国生物制品学杂志2010年9月第23卷第9期 Chin J Biologicals September20l0,Vol.23No.9
中国图书分类号S852.4文献标识码A文章编号1004-550301009-099-03
治疗制品
猪、犬脾转移因子的理化性质及生物学特性分析
孔庆波
【摘要】目的分析猪、犬脾转移因子( Transfer factor. TE的理化性质及生物学特性。方法分别对猪、犬脾TF提取物进
行性状、pH值、蛋白质、氨基酸组分检测、禁外光谱分析及TF、DNA、核苷酸含量测定等理化性质分析,并进行无菌试验、热原试
验、安全试验、过敏试验、免疫原性检測等生物学特性分析及生物学活性检测。结果提取的猪、犬脾 TF DNA、核苷酸、氨基酸含
量不同,氨基酸组分也有差异,二者均无菌,无热原、毒性、致敏性及免疫原性,且Ax020值也达到较高的指标,未黏附白细胞抑
制指数(NAD均大于20%,猪、犬脾TF每毫升约为2个活性单位。结论提取的猪、犬TF的理化性质及生物学特性与国内外报
道的其他TF相符,已达到国内外同类制品的水平,可供试验和临床使用。
【关键词〕猪;犬;脾;转移因子;理化性质;生物学特性
Physico-chemical Properties and Biological Characteristics of Swine and Ca
nine Spleen Transfer Factors
KONG Qing-bo Police Dog Technique School of The Ministry of Public Security, Shenyang 110034, Chind
Abstract Objective To analyze the physieo-chemieal properties and biological characteristics of swine and canine spleen
transfer factors (T'ES. Methods The TF extracts from swine and canine spleens were observed for appearance, determined for PH
value as well as protein and amino acid composition, subjected to ultraviolet spectroscopy, analyzed for TF, DNA and nucleotide con
tents, then subjected to sterility, pyrogen, safety, sensitivity and immunogenicity tests and determined for biological activity. Results
The DNA, nueleotide and amino acid contents as well as amino acid compositions of TF extracted from swine spleen were diferent
from those from canine spleen. However. both swine and canine spleen TFS were qualified in sterility, pyrogen, toxicity and sensitivity
tests, showed no immunogenicity and reached high A 20, 0 values. Both the non-adherence indexes of the Tps were more than 20%
Each milliliter of swine and canine spleen TES contained about 2 activity units. Conclusion The physico-chemical properties and
biological characteristics of extracted swine and canine spleen TP were consistent with those of other TPS reported in China and other
countries, indicating that both the qualities of the Tis reached the levels of domestic and imported products of the same kind, which
was suitable for laboratory and clinical uses
Key words] Swine; Canine; Spleen; Transfer factor; Physico-chemical properties; Biological characteristics
转移因子( Transfer factor,TF)是从淋巴细胞中
目前,TF种类较多,从免疫特性上可分为特异
提取的一类低分子多肽与核苷酸复合物。其作为ー性"TF和非特异性"F两大类。其中非特异性T已
种特定的淋巴因子,具有传递免疫信息、激发免疫细大量应用于临床,可非特异性调节机体的免疫机能
胞活性、调节和増强机体特异性及非特异性细胞免从而提高机体的抵抗力或疫苗的免疫效果。实践证
疫的功能,促进B细胞的抗体分泌及诱导机体产生明,该制剂对人体安全无毒,无药物代谢残留,口服
干扰素等生物活性。F能将供体的细胞免疫功能特或注射同样具有免疫活性。近年来,我国已陆续开展
异地转移给受体,受体接受TF后,很快②~24产了对畜禽TF的研究和应用,先后研制了猪、羊、马、
生效应,并可持续数月至1年。这种效应无严格的种兔、牛、鸡及犬的非特异性TF和特异性TF[5,在畜
属特异性;其能转移多种抗原的细胞免疫,包括禽疾病的防治上取得了良好效果。本文对采用常规
真菌、细菌、病毒、寄生虫、组织相容性抗原和肿瘤抗方法提取的猪脾TFTF-D和犬脾TF(CS-TD的理
原,是一种新型免疫增强制剂,可用于当前用抗化性质及部分生物学特性进行了分析比较,为其防
菌素或免疫球蛋白无法控制的某些细胞内感染性疾治动物疾病提供依据。
病的防治,对某些免疫缺陷病也展示出广阔的应用
前景
1.材料与方法
1.1TF
基金项目:公安部科技局基金项目(2004582950
作者单位:公安部警犬技术学校(沈阳1100340
猪、犬脾"F由西北农林科技大学动物医学院免
通讯作者:孔庆波,D-mail:kgl1170163.com
疫实验室提取。