大孔树脂纯化东北山核桃壳棕色色素工艺的研究.pdf

食品研究与开发
2016年8月
天然产物
Food Research And Development
第37卷第16期
27
DOI:10.39695-issn.1005-6521.2016.16.030
大孔树脂纯化东北山核桃壳棕色色素
工艺的研究
秦微微,金婷,王爽,宋学东,丁振铎,张衡
(1.黑龙江生态工程职业学院,黑龙江.哈尔滨150025:2.枣庄学院生命科学系,山东枣庄27160;3.哈尔
滨市农业信息中心,黑龙江哈尔滨150021)
摘要:为减少东北山核桃壳棕色色素的杂质,提高其色价,选择4种大孔吸附树脂,分别测定了它们对棕色素的吸附
和解吸率,筛选出较优的棕色素吸附剂,并对其吸附性能进行了考察。结果表明:NKA-9树脂对棕色素有较好的吸附和解
吸效果,当浓度为O.1 mg/mi.时树脂的吸附效果最好,上样液的最佳pH值为3.0,最佳解析液为70%乙醇溶液,最佳上样液
流速为2.0ml/min,最佳洗脱液流速为1. mi/min,洗脱剂用量不少于3BV经树脂纯化后,色素色价可提高至162.81%
关键词:大孔树脂;纯化;核桃壳;棕色素
Study on Purification Process of Resin to Northeast Walnut Shell
QIN Wei-wei, JINTING, WANG Shuang, SONG Xue-dong DING Zhen-duo', ZHANG Heng
(I. Heilongjiang Vocational Institute of Ecological Engineering, Harbin 150025, Heilongjiang, China; 2. Iife
Science Department of Zaozhuang University, Zaozhuang 277160, Shandong, China; 3.Agricultural Information
Center of Harbin City, Harbin 150021, Heilongjiang, China)
Abstract: In order to reduce the impurities of brown pigments from northeast walnut shell and improve its colo
value, type of macroporous resin for purification of pigments was studied and the purification process conditions
was researched. The characteristics of adsorption rates and desorption rates of four kinds of macroporous resins
(D-101, D-4020, AB-8, NKA-9 )to the brown pigment are investigated. The results showed that NKA-9 resin
was the suitable resin for purifying the brown pigment. From the above investigations and analysis, the optimized
conditions were oblained as O I mg/m sample concentration of adsorption, PH value 3.0, 70%o alcohol of the
suitable elution agent, the optimized flow rates of adsorption were 2.0 mlmin, the optimized flow rates of des
orption were 1.0 ml/min, the consumption of elution agent werent less than 3 V After purification, purification
rate of color value was ahieved 162.81
Key words: macroporous resin; purification; walnut shell; brown pigment
核桃壳属丁山核桃加工的废弃物,在黑龙江省林其粗提液中含有糖类、蛋白质等杂质,为了提高棕色
地面积可达1962.13万公顷,山核桃的产量非常丰富。素的品质,拓宽其应用范同,本文对粗提液进行了纯
随着核桃产品加工量的逐年扩大,核桃売的利用引起化研究
了人们的关注。对核桃壳的深入研究发现,核桃壳中
大孔吸附树脂具有理化性质稳定,不溶于酸碱和
含有天然的棕色色素,将这种天然色素提取出来,并有机浴剂,选择性好,不受无机盐的影响,解吸温和、再
用于食品加工中,既避免了核桃売资源的浪费,又满生简便、使用周期长等优点。自20世纪70年代以来
足了人们对健康、安全的天然色素的需求,充分地体大孔树脂分离技术被广泛用于天然产物分离。目前,
现了核桃売的价?。核桃売棕色色素在提取过程中,利用大孔树脂纯化东北山核桃売的报道很少,本文就
基金项口:黑龙T省森林木T业总201年科技计划顶口(Y20100大孔树脂纯化山核桃売标色色素的工艺进行了研究
作者简介:秦微微(1980-),女(汉),副教授,倾⊥,研究方向:食品旨在为东北山核桃壳棕色色素的深人研究奠定一定
检测
的理论参考。