高血压患者尿微量清蛋白与C反应蛋白及其-717A〉G基因多态性的关系.pdf

(GIP Chinese General Practice
270
January 2012, Vo 15 No 1C
论著
高血压患者尿微量清蛋白与C反应蛋白及其-717A>G基因
多态性的关系
吴建华,张定式,崔之础,王锋,黄新忠,范亚平
摘要】目的探讨高血压患者尿微量清蛋白(MAU)与超敏C反应蛋白(hs-CRP)及其-717A>G基因多
态性的关系。方法将高血压患者接尿清蛋白水平分为尿正常清蛋白(NMAU)组(75例)和MAU组(56例),另
选择健康体检正常者为对照组(60例)。免疫浊度法测定各组血浆hs-CRP水平,聚合酶链反应一限制性片段长度多
态性(PCR-RFIP)技术检測受检者CRP的-717A>G基因型及等位基因频率,并进行组间比较。结果3组受检者
的收缩压、舒张压、hs-CRP、MAU、总胆固辞(TC)、三酰甘油(TG)水平比較,差异均有统计学意义(P<
0.05)。其中NMAU组与MAU组的收缩压、hs-CRP、MAU、TG水平比较,差异均有统计学意义(P<0.05)。 Logis-
tic回归分析显示hs-CRP升高是高血压患者MAU发生的危险因素(OR=3.107)。MAU组与NMAU组受检者比较,
CRP-717A>G基因型A、AG及GG频率分布差异无统计学意义(2=3.621,P>0.05);等位基因A、C频率分布
差异有统计学意义(x2=3.931,P=0.047)。结论hs-CRP升高是高血压患者MAU发生的独立危险因素,CRP
717A等位基因可能是高血压患者MAU发生的易患基因。
【关键词)高血压;尿微量清蛋白;C反应蛋白;多态性,单核苷酸
【中图分类号)R544.1【文献标识码】A【文章编号】1007-9572(2012)01-0270-03
The Sociation of M bmin wt Crate Potein and S-717A > G Polymorphism in Hypertensive Pa
tients Wuian-hua, ZHANG Ding -wu, CU Zhi-chu, t al. Department of Nephrology, Afiliated Hospital of Nantong
University, Nantong 226001, China
Abstract] Objective To investigate the association of microalbuminuria( MAU) with high-sensitivity C-reactive
protein(hs-crp) and its-717a >G gene polymorphism in hypertensive patients. Methods Hypertensive patients were divid-
ed into urine normal albumin(NMAU )group(75 cases and MAU group(56 cases by urinary albumin. 60 healthy volunteers
served as controls. The serum levels of HS-CRP of the subjects were measured, and the CRP-717A > G genotypes and allel.
were screened by polymerase chain reaction-restriction fragment length polymorphism(pcr-rflp).All those results were
compared between groups. Results The differences of systolic blood pressure, diastolic blood pressure, HS-CRP, MAU, TC
and TG between the three groups were statistically significant(P<0.05).The concentrations of HS-CRP, MAU, triglyceride
(TG and systolic blood pressure in the MAU group was higher than those in NMAU group(P <0.05). Logistic analysis showed
that higher HS-CRP was the risk factors of MAU in hypertension patients(OR=3. 107). There were no significant differences
in the frequency of CRP-717A >G genotype between the NMAU and MAU groups(x=3. 621, P>0.05).And the frequency
of CRP-717A allele in the MAU group was higher than that in NMAU group(X=3.931, P=0. 047). Conclusion Higher
HS-CRP was the independent risk factor of MAU in hypertension patients. CRP-717A allele may be a susceptibility gene of
MAU in hypertensive patients
Key words Hypertension; Microalbuminuria; C-reactive protein; Polymorphism, single nucleotide
尿微量清蛋白( microalbuminuria,MAU)是原发性高血脑血管事件的独立预测因子。高血压的发生发展具有一定
压(EH)患者肾脏损害的预测指标,也是高血压患者发生心的遗传易患性及家族聚集性,炎症在这一过程中扮演了重要角
色。本硏究通过检测不同高血压患者炎症标记物超敏C反应
基金项目:南通市科技项目(S2009062
蛋自(hs-CRP)水平及其-717A>C基因多态性,探讨高血
作者单位:226001江苏省南通市,南通大学附属医院肾内科(吴
压患者MAU与hs-CRP及-717A>C基因多态性的相关性。
建华,张定武,王锋,黄新忠,范亚平),酶学实验室(崔之础
通讯作者:张定武,226001江苏省南通市,南通大学附属医院肾
1资料与方法
内科;E-mail:ntyyzhang@163.com
1.1一般资料选取2009年11月-2010年1月在我院门诊
及住院尿常规检查蛋白阴性的高血压患者131例为研究对象