响应面法优化甘露醇产丁醇的发酵条件.pdf

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响应面法优化甘露醇产丁醇的发酵条件
李锋2,温顺华,黄庶冰3,吴卫红2,廖威4,王何健5,黄庶识
(广西科学院生物物理实验室,广西南宁530007:2广西科技大学生物与化学工程学院,广西柳州5456006:
广西大学商学院,广西南宁5300044广西职业技术学院食品与生物技术系,广西南宁530226:S广西科学
院国家非粮生物质能源工程技术研究中心,广西南宁530007
摘要:以褐藻的主要成分甘露醇为底物,利用丙酮丁醇梭菌ATCC824发酵生产丁醇。采用 Plackett-burman(P-B
和Box- Behnken优化发酵条件。在 Plackett-burman实验设计时,考察牛肉膏、酵母浸粉、胰蛋白胨、乙酸铵、
KH2PO4、MgSO4:7H12O、FeSO47H2O、接种量、发酵温度、初始pH值这10个因素对丁醇产量影响,筛选出影
响丁醇发酵的重要参数是发酵温度、初始PH值、乙酸铵浓度,利用Box- Behnken设计确定最优发酵条件为发酵
温度37,3℃、初始pH值6.38、乙酸铵浓度2.82gL。数学模型分析预测最大丁辞产量为8.47gL.经实验验证
表明,在优化条件下的丁醇产量平均值达到8.20.55gL。这说明利用统计学方法优化甘露醇的丁醇发酵条件是
有效的
关键词:甘露醇;丁醇; Plackett-burman;Box- Behnken;两酮丁醇梭茵
中图分类号:TQ923
文献标志码
文章编号:1000-6613(2013)07-1629-06
DOl:10.3969j.iss:.1000-6613.2013.07.030
Optimization of butanol production from mannitol by Clostridium
acetobutylicum ATCC824 using response surface method
LI Feng", WEN Shunhua, HUANG Shubing, WU Weihong", LAO Wei", WANG Hejian
HUANG Shushi
Lab of Biophysics, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China; "School of Biological and
Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi, China; Business
School, Guangxi University, Nanning 530004, Guangxi, China; "Department of Food and Biotechnology, Guangxi
Vocational Technical College, Nanning 530226, Guangxi, China; National Engineering Research Center for non-food
Biorefinery, Guangxi Academy of Sciences, Nanning 530007, Guangxi, China
Abstract: Mannitol, which is the main component of brown algae, was utilized in this study to produce
butanol by Clostridium acetobutylicum(ATCC 824). Plackett-burman(P-b )and Box-behnken designs
were adopted to optimize the fermentation conditions. Ten factors, including beef extract, yeast
extract, tryptone, ammonium acetate, KH2PO, MGSO47H2O, FESO4 7H20, size of inoculum
fermentation temperature and initial ph, were considered to affect butanol production in the
Plackett-burman( P-B )design. The result demonstrated that fermentation temperature, initial PH and
concentration of ammonium acetate were the most critical factors, and optimal fermentation conditions
were 373C, PH 6.38 and 2. 82 g/L of ammonium acetate according to the Box-behnken design. A
mathematical model was established and used to predict the maximum butanol yield of 8.47 g/L. The
收稿日期:2012-12-26:修改稿日期:2013-01-21
第一作者:李锋(1982-),男,士研究生,研究方向为生物质能
基金项目:广西自然科学基金重点项目(201OGXNSFD013029)及广源。E-mailhuagonglflf163.com。联系人:黄庶识,研究员,主要从
西科学院基本业务费项目(10YJ25WL01,12YJ25WL12)。
事主要从事生物质能应用技术研究工作。E-mailshushi@cgxa.cn。