rhBNP对慢性心力衰竭大鼠心室重构及心功能的保护意义.pdf

3466
重庆医学2015年9月第44卷第25期
论蕃?基础研究di:10.3969/.issn.1671-8348.2015.25.004
hBNP对慢性心力衰竭大鼠心室重构及心功能的保护意义
钟小兰,景江新?,班努?库肯,马依形3
(1.新疆医科大学第二附属医院心内科,乌鲁木齐830063;2.新疆医科大学第二附属医院超声室
乌鲁木齐830063;3.新疆医科大学第一附属医院心脏中心,乌鲁木齐830054)
摘要]目的观察重组人脑利钠肽( THBNP)对慢性心力衰竭大心室重构及心功能的影响。方法使用阿雾素诱导慢性
心力衰竭大鼠模型,用不同剂量 RHBNP治疗,4周后检测相关指标。结果经 RHBNP治疗的心カ衰大泉中,中、高剂量rhBN
组左心室质量、左心室质量指数均缩小,左心室长短轴比値增大,但两組比较差异无统计学意义(P>0,05)。经 RHBNP治疗的心
力衰媧大鼠較心力衰竭对照组心功能指标普遍好转,其中以高剂量 THBNP组的收编压、舒张压、左心室收縮压、左心室内压最大
上升和下降遠度明显升高,左心室舒张末压明显下降(P<0.05)。 RHBNP治疗后的大鼠血清、心肌肿瘤坏死因子a(TNF-a〉/IL-
10水平普遍出现下降/升高(P<0.05)。结论长期使用 RHBNP对慢性心力衰竭大鼠心室重构及心功能具有保护作用,其机制
可能与 RHBNP对炎症细胞因子网络的正向调控作用相关。
关键词]心室重构;炎性因子;重组人脑利钠肽;心力衰竭
[中图分类号]R541
文献标识码]A
[文章编号]1671-8348(2015)25-3466-04
Protective effects of RHBNP on left ventricular remodeling and heart function in chronic heart failure rats
Zhong Xiaolan Jing Jiangxin 4, Bannu Kuken, Ma Yitong
(1. Department of Medical Cardiology, the Second A ffiliated Hospital of Xinjiang Medical University
Urumqi, Xinjiang 830063, China; 2. De partment of Ultrasonogra phy, the Second Affiliated Hospital of
Xinjiang Medical University, Urumqi, Xinjiang 830063, China; 3. The Heart Center of the First Affiliated
Fos pital of Xiniiang Medical University, Urumgi, Xinjiang 830054, China.
I Abstract]Objective To explore the influence of recombinant human brain natriuretic peptide(RHBNP) on the ventricular re
modeling and cardiac function of chronic heart failure rats. Methods Doxorubicin was injected for 2 weeks to obtain the chronic
heart failure rats. Then different doses of RHBNP were injected for 4 weeks and objective indexes were recorded. Results The inde
xes were generally improved in the rats with RHBNP injection. The left ventricular absolute weight and left ventricular mass index
obviously decreased in both middle and high dose group, but none difference was found between the two groups(P>0.05). The in
dexes of cardiac function of the high dose RHBNP group were improved most obviously, with the systolic pressure, diastolic pres-
sure, left ventricular systolic pressure increasing and left ventricular end-diastolic pressure decreasing right ventricular and diastolic
stage decreased significenfly(P=0. 05). The serum and myocardial levels of TNF-A /IL-10 generally decreased/increased in the rats
after RHBNP treatment(P<0. 05) Conclusion Long-term RHBNP treatment is likely to protect the ventricular remodeling and car-
diac function and of chronic heart failure, which maybe relative to the ositive regulation on inflammatory cytokine network of rhb
[Key words] ventricular remodeling; inflammatory factor; RHBNP heart failure
曼性心力衰竭( chronic heart failure)是一个心脏功能逐渐1材料与方法
减退的过程,一且出现心力衰竭,即使去除原发病因,心脏仍会1.1实验动物及主要试剂 SPF K SD雄性大鼠共50只,体
由于心力衰竭引起的心肌重构而导致心功能进行性下降い2?,质量200~250g,购自重庆医科大学实验动物中心。动物在恒
而临床中慢牲心カ衰竭以左心室衰竭常见。重组人脑利钠肽温实验室(22士2)℃适应3d后开始实验。 RHBNP购自西疲药
( recombinant hurnan brain natriuretic peptide, RHBNP)作为利业成都诺迪康生物制药有限公司,蛋白纯度大于95%,20℃
钠多肽家族(心钠素、脑利钠肽、C类利钠素)中的一员,有利保存,使用时临时用0.9%氯化钠溶液配制成相应浓度。肿瘤
钠、利尿、扩张血管等作用,短期应用治疗急性心力衰竭,可明坏死因子a(TNF-a)、L-10 ELISA试剂盒购自上海西唐生物
显改善患者血流动力学指标从而改善心力衰竭症状「。近期科技有限公司
研究[发现其可改善心肌梗死后心力衰竭的心室重构及心功1.2方法
能。由此,本文探讨hBNP是否可改善其他病因所致心力衰1.2.1慢性心力衰竭大鼠模型的建立及分组参照文献方法
竭的心室重构及心功能情况,如阿霉素诱导的心力衰竭,为临用阿霉素诱导建立慢性心力衰竭大鼠模型。将大鼠分成健
床长期使用 RHBNP治疗慢性心力衰竭打下基础
康对照组、心力衰竭对照组(阿霉素诱导)低剂量 RHBNP治疗
*基金项目