近红外光谱快速测定 Mortierella alpina菌丝体油脂含量.pdf

2011年第36卷第1期
中国油脂
77
检测分折
近红外光谱快速测定 Mortierella alpina
菌丝体油脂含量
潘安龙,王品',张贺',邹贤勇2,徐昆3,薛冬梓
(1.长春エ业大学化学与生命科学学院,长春130012;2.瑞士步珙实验室(上海),上海20002;
3.中国科学院长春应用化学研究所,长春13002
摘要:为实现玉米皮渣发酵生产微生物油脂过程中茵丝体油脂含量的实时监测,建立了菌丝体油脂
含量测定的近红外定标模型。结果表明,采用偏最小二乘法(PIS)、归一化预处理方法,模型的定
标集相关系数(R)及验证集相关系数分别为0.9974和0.9058,定标集标准偏差(SEE)为1.2666,验
证集标准偏差(SEP)为1.4171,定标集标准偏差(SEE)与验证集标准偏差(SEP)的比值为
0.8937,这表明该方法可快速准确地测定菌丝体油脂含量。
关键词:近红外光;玉米皮渣;菌丝体;油脂含量;模型;徽生物油脂
中图分类号:TQ646;0657
文献标志码:A
文章编号:1003-7969(201)01-0077-03
Rapid determination of microbial oil content in mycelium of
Mortierella alpina by near-infrared spectroscopy
PAN Anlong, WANG Jing, ZHANG He", ZHOU Xianyong
XU Kun, XUE Donghua
(1. School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012
China; 2. Buchi Laboratory Equipment Trading(Shanghai), Shanghai 200052, China
3. Changchun Institute of Applied Chemistry, CAS, Changchun 130022, China)
Abstract: The near-infrared spectrum model for determination of microbial oil content in mycelium was
set up so as to realize the reall -time detection of the oil content in mycelium during the microbial oil pro-
duction from corn fiber by ermentation with Mo erella alpina. The partial least square regression(PLS
and the spectral data pretreatment of normalization were used to analyze the model. The results showed
that the correlation coefficient(R)of calibration set(c-set )and validation set(v-set)were 0.9974
and 0.995 8, respectively, standard error of calibration set(SEE)was 1.2666, standard error of cross
validation(SEP) was 1. 417 1, the ratio of SEE to SEP was 0.8937. The method could measure the oi
content in mycelium rapidly and accurately
Key words: near-infrared spectroscopy; com fiber; mycelium oil content; model; microbial oi
玉米皮渣(玉米粒的细胞壁组分)是玉米湿磨法制备淀粉过程中产生的副产物,玉米皮渣中纤维
素、半纤维和淀粉等生物质资源含量达70%以上。
收稿日期:2010-07-06;修回日期:2010-11-10
采用酸酶混合水解技术,可以获得可发酵性单糖,用
基金项目:国家自然科学基金(5063089);吉林省教育厅重于 Mortierella alpina菌丝体发酵生产微生物油脂。
点项目基金资助(2008100;2009121)
微生物油脂又称单细胞油脂是指某些微生物在
作者简介:潘安龙(1984),男,硕士研究生,有机化学专业
(e-mail)anlong107@yahoo.com.cn,
定条件下将碳水化合物、碳氢化合物和普通油脂
通信作者:薛冬榉,教授,博士(Ema) donghua mail.等碳源转化为菌体内储存的油脂”。微生物油脂
ccut. edu. cn
可生产功能性油脂,并可代替植物油制备生物柴油。
万方数据