重组人生长激素对裸鼠胃癌移植瘤生长及血清VEGF浓度的影响.pdf

南太学学报(医学版)2010,Jun;29(3):291-295
J Southeast Univ( Med Sci Edi )
291
论著
重组人生长激素对裸鼠胃癌移植瘤生长及
血清VEGF浓度的影响
程路,林岩,李苏宜
(1.东南大学医学院,江苏南京210009;2.南京医科大学第二附属医院,江苏南京2100]1
摘要]目的:研究重组人生长激素(rhGH)对课鼠人胃癟移植瘤生长及血清VECF水平的影响。方法:取60
只雄性褓鼠进行实验,通过免疫细胞化学染色法检测胃癌细胞株SCC-7901及MKN-45生长激素受体
(GHR)的表达状况,将I照胆性表达的罚癌细胞株SGC-7901及CHR阴性表达的胃癌细胞株MKN-45分
别接种于4只裸鼠皮下,制作裸泉胃癌威下移植癘模型,3周后取出移植制成组织匀浆,接种于剩余52只
课鼠。依据接种细胞种类不同分为移植瘤CHR阳性表达和CHR阴性表达棵鼠,不同CHR表达的棵鼠进一
步分为对照组(C组:予0.9%NaCl,0.2mld')、rhGH低剂量组(L组:予rhGH0.5U?kg-'?d-')、rhGH高
剂量组(H组:予rhGH2.5U?kg?d'),连续给药14d,观察并记录各组课鼠体重和肿瘤体积变化,酶联免
憂吸附法测定血清VECド量。结果:SCC-7901为CHRF性表达的人胃癌细胞株,MKN-45为CHR阴性表
达的人胃癌细胞株。对于接种GHR阳性表达的人癌细胞株的裸鼠,hGH给药组皮下移植瘤体积较对照
组增大(P<0.05),且H组促増长效应显著(P<0.05),3组间体重差异不明显(P>0.05);血清VECF检
冽,.组为(167.60±9.54)pg“ml,H组为(252.94±15.32)pgml,较C组(49.94±5.73)Pg·ml升
高(P<0.05);对于接种GHR阴性表达的人屑癌细胞株的棵鼠,L组、H组体重均大于对照组(P<0.05),而
3组之间肿癌体积大小、血清VEGF水平比较差异均无统计学意义(P>0.05)。结论:hH能促进CHR阳
生表达的胃癌细胞株SCC-7901移植寤生长,并促进血清VECF表达増高;对GHR阴性表达的胃癌细胞梾
MKN-45移植没有表现出明显的促肿瘤生长及促血清VECF表达效应。GHR表达情况可能是hGH影响血
清VECF分汝的关犍靶点之
[关键词」重组人生长激素;生长激素受体;血管内皮生长因子;胃癌
L中图分类号」R-33;R736.2[文献标识码A{文章编号1671-6264(2010)03-0291-05
doi:10.3969/j.isa.1671-6264.2010.03.013
Effects of recombinant human growth hormone on tumor growth and serum
VEGE concentration of human gastric carcinoma xenografts in nude mice
CHENG Lu!. LIN Yan, LI Su-yi
(I School af Medicine, Southeast University, Nanjing 210009, China; Department of Oncology
the econd Affiliated Hospital e ann Medc u lers, uning 210011, China y
Abstract Objective: To investigate the effects of recombinant human growth hormone( RHGH) on tumor growth
and serum VEGF levels of human gastric carcinoma engrafts in nude mice. Methods: Si]ly male nude mice were
收稿?期1200912
修回日期]2010-01-20
基金项目」南京市医学科技发展项日(YKO7097)
[作者简介」程路(1984-),女,江ぶ南京人,医学硕上,研究方向为恶性肿揺的代谢支持与代谢调埋。E-mail:decheng007@163.com
「通讯作者」李苏宜E-mil:lisuyic@cro.rg.cn
东南大学学报(医学版)2010年6月,29(3
ought for the experiment. Immunocytochemistry nethod was used to deteet the expression of growth hormone
recep or(GHR)in hurnan gastric careinoma cell lines SGC-7901 and MKN-45. Human gastrie eel line SGC-7901
with a positive expression of GHR and human gastric cell line MKN-45 with a negative expression of GHR were
injected subcutaneously into 4 nude mice respectively to create the subcutaneous xenografts models derived from
human gastric carcinoma in nude mice. Three weeks later, the xenografts were picked out to make homogenate. Then
injected subcutaneously into the remaining 52 nude mice Accordin to the different types of the injected cells, th
mude mice were divided into GHR positive group and GHR negative group, then divided into three experimental
groups: control group(C grup: 0. %o Nacl was given 0. 2 mld ), low-dose RHGH group(L group: RHGH was
given 0.5 U. d ),and high-dose RHGH group(H group: RHGH was given 2.5 U.. d ),once a day for
14 days. The changes of body weight and tumor volume of nude mice in each group were recorded. The serum
concentrations of VEGF in peripheral blood were analyzed by ELISA. Results: We efined SGC-7901 as GHR
positive
RHGH administration groups than in the C group(P<0.05), and the I group revealed greater effect(P
0.05) No significant difference was found in body weight of the mude mice among the 3 groups(P>0.05). The
ser