肾移植术后慢性排斥患者外周血Th17细胞的表达.pdf

第33卷第1期
中山大学学报(医学科学版)
Vol33 No.1
2012年1月
JOURNAL OF SUN YAT-SEN UNIVERSTTY(MEDICAL SCIENCES
Jan.2012
肾移植术后慢性排斥患者外周血Th17细胞的表达
张圣平,纳宁,傅茜2,洪良庆?,黄正宇,缪斌,赵大强,李衡
(中山大学1.附属第三医院肾移植科,广东广州510630;2.附属第一医院器官移植中心,广东广州510080
摘要:【目的】探讨外周血T17细胞及相关细胞因子与肾移植术后慢性排斥反应发生的关系。【方法】自2007年1月
至2010年8月,在中山大学附属第一医院和第三医院收集尿毒症(尿毒症组)、肾移植术后6个月内未发生慢性排斥反应
(非慢排组)及6个月内发生慢性排斥反应(慢排组)患者的外周血共75例,其中每组各25例。采用流式细胞术(FCM)检测
外周血中CD4T细胞中Thl7细胞的频率。采用酶联免疫吸附法( ELISA)检测血清中Th7细胞相关细胞因子白介素-17(T
17)、白介素-22(IL-22)和白介素-23(L-23)的水平。分析比较组间差异。【结果」】术后慢排组Tl7细胞的频率为(3.8±
1.7)%,明显低于非慢排组(22.0士11.0)%和尿毒症组(17.2±8.0)%,差异均有统计学意义(P<0.01)。肾移植术后慢排组患
者产生L-17的量为(7.8±1.3)pg/mL,明显低于非慢排组(17.0±6.0)pg/mL(P<0.05)和尿毒症组(20.4±9.0)pg/ml(P<
0.01),差异均有统计学意义。L-22和I-23在3组间差异无统计学意义(P>0.05)【结论】外周血中1h17细胞可能通过分
泌细胞因子L-17参与了肾移植术后慢性排斥反应的发生
关键词:肾移植;慢性排斥;Th17细胞;白细胞介素17;白细胞介素22;白细胞介素23
中图分类号:R692.5
文献标志码:A
文章编号:1672-3554(2012)01-0102-05
Expression of Peripheral Blood Th17 Cells in Patients with Chronic Rejection after Kidney
Transplantation
ZHANG Sheng-ping NA Ning, FU Qian, HONG Liang-qing", HUANG Zheng-yu
MIU Bin, ZHAO Da-qiang L Heng
(1. Department of Kidney Transplantation, Third Affitiated Hospital, Sun Yat-sen University, Guangzhou 510630, China
2 Department of Organ Transplantation Iirst Affiliated Ilospital, Sun Yat-sen University, uangzhou 510080, China)
Abstract [Objective To investigate the association between the expression of peripheral blood Th17 cells, relevant cytokines
in blood and the chronic rejection afler kidney transplantation. [Methods] From January 2007 to August 2010, a total of 75 samples
were collected in First and Third Affliated Hospital, Sun Yat-sen University, including patients with uremia(PU group, non-chronic
rejection in six month after kidney transplant NCR group), and chronic rejection in six month after kidney transplant (CR group)
and 25 patients for each group. The expression of peripheral blood Th17 cells were measured by FCM and interleukin-17(il-17)
interleukin-22(11-22, and interleukin-23(i-23 ) levels were evaluated by ELISA. Then we analy zed the differenees between three
groups. Results The expression of Th17 cells in chronic rejection group was(3.8+1.7)%, which was significantly lower than those
in uremia group (22.0+ 11.0)% and non-chronic rejection group(17.2 +8.0)%(P values <0.01). The Ir-17 levels in chronic
rejection group were (7.8*1.3)pg/ml, which were also significantly lower than those in uremia group (20.4#9.) /ml(P<
0.01) and non-chronic rejection roup(17 #6.0)pg/ml(P<0.05) However, there were no statistically significances in thre
groups with respect to the levels of IL-22 and IL-23 (P values > 0.05). [Conclusion] In peripheral blood, Th17 cells might play
important role in the activation of the chronic rejection after kidney transplantation through increased secretion of IL-17
Key words: kidney transplantation; chronic rejection; systemic helper 17 cell; IL-17; IL-22: IL-23
IJ SUN Yat-sen Univ(Med Sci), 2012, 33(1): 102-106
收稿日期:2011-07-0
基金项目:广东省科技计刘项目(2007B031500014
作者简介:张圣平,硕士研究生,研究方向:泌尿外科学,E-mail:docpingol@163.com;*通信作者:洪良庆,副教授,硕士生导师,E-mail
horselq(@163.com