IFN-γ对兔盆腔术中照射后直肠组织TGF-β1mRNA表达的影响.pdf

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MODERN ONCOLOGY, Feb 2010, VOL 18, NO. 2
7]王军,宗志红,欧绍武,等编码脑组织Navl.5钠通道新外显子[13] Ross SB, Fuller CM, Bubien K,etal. Amiloride- sensitive Na
的克降、鉴定和分布しJ」.生物化学与生物物理进展,2007,34
channels contribute to regulatory volume increases in human glio-
(3):255-259
na cells J]. Am J Physiol Cell Physiol, 2007, 293(3): C1181
[8]欧绍武,宗志红,王军,等.Nawv!.5/SCN5A基因新的变构体编
码人脑组织Navl.5Nat通道[J!生物化学与生物物理进展,[14] Weiss RE, Sidel N. Sodium currents during differentiation in
human neuroblastoma cell linel J].J Gen Physiol, 1991, 97(3
[9] Brackenbury WJ, Chioni AM, Diss JK, et al. The neonatal splice va
rant of Navl. 5 potentiales in vitro invasive behaviour of MDA
15] Olsen ME, Schade S, Lyons SA, et al. Expression of voltage-gated
MB-231 human breast cancer cells[ J Breast Cancer Res Treat
chloride channels in human glioma cells JJ. J Neurosci, 2003, 23
2007,101(2):149-160
13):5572-558
[10] Wu WK, Li GR, Wong HP, et al, on channels and amino acid [16 Dueret T, Vacher AM Vacher P Voltage-dependent ionic con
transporters support the growth and invasion of primary brain
ductance in the human malignant astrocytoma cell line U87-MG
tumors [J]. Mol Neurobiol, 2005,29(1): 61-71
Mol Membr Biol,2003,20(4):329-343
[11 Schrey M, Codina C, Kraft R, et al. Molecular chara
of .17vila-camiles WH, K
CG. Jovoy B et al
voltage-gated sodium channels in human gliomas J]. Neurore
of AS[C2 inhibits the amiloride-sensitive current and migration
2002,13(18):2493-2498.
of glioma cells[ J]. J Biol Chem, 2006, 281(28): 1
[12] Kawaguchi A, Asano H, Matsushima K, et al. Enhancement of so- [18] Wang WX, Ji YH. Scorpion venom induces glioma cell apoptosis
dium current in NG108-15 cells during neural differentiation is
in vivo and inhibits glioma tumor growth in vitro[J]. J Neuroon
mainly due to an increase in Nav1. 7 expression [J]. Neurochem
col,2005,73(1):l-7.
Res,2007,32(9):1469-1475
(编校:张志明
IFN-对兔盆腔术中照射后直肠组织TGF-B1mRNA表达的影响
张丽,王华2,王娟,杨蕴一,刘孜
Effect of inteferon y in introoperative radiotherapy induced-rectall TGF-B1 MRNA in-
jury of experimental rabbits
ZHANG Li, WANG Hua", WANG Juan, YANG Yun-yi, LIU Zi
Department of Radiation ncology, the First Afil ted Hospital of Medical School of Xian otong University, Xian 710061, China
2 Xian Gaoxin Hospital. Xian 710075 China
Abstract] Objective: To observe the expression of transforming growth factor1(TGF-B1)changes in rectum
tissue of rabbis with introoperative radio hera(IORT induced-injury for exploring the preventive effect of inter-
feron -gamma(ifn-y). Methods: Fifty four big albino rabbits were randomly allocated into the simple irradiation
group(n=25), the IFN-Y treatment group(n=25 )and the blank control group(n=4). The rectum of all rabbits
except those in the blank control group were IORT, total of 20GY. Five rabbits in the two groups were randomly sacri-
ficed at each of the 5 time points(4, 8, 12, 16, and 20 weeks after IORT), their rectum was harvested out, sliced and
dyed with stain, hybridization in situ, then the levels of TGF-B1 MRNA expression in the rectum tissue were ob-
served by hybridization in situ. Results: The most serious acute factitial otitis was seen in the 4h week and rectal
fibrosis was remarkable in the 20th week and the levels of TGF-BI MRNA expression were enhanced in