免疫亲和净化-光化学衍生-高效液相色谱法检测动物肝脏中六种黄曲霉毒素.pdf

第5卷第3期
食品安全质量检测学报
VOL 5 No. 3
2014年3月
Journal of Food Salcly and Quality
Mar..2014
免疫亲和净化-光化学衍生-高效液相色谱法
检测动物肝脏中六种黄曲霉毒素
陈冬东,代汉霞,彭涛,姚佳2,王昕2,张建新2,里南,王雄
(1.中国检验检疫科学研究院,北京10025:2.西北农林科技大学,杨凌712100
3.北京中检维康生物技术有限公司,北京10004
摘要:目的以鸡、猪等动物肝脏为研究对象、二氯甲烷为提取溶剂、建立免疫亲和柱净化、高效液相
色谱-光化学衍生荧光检測器检测六种黄曲霉毒素的分析方法。方法样品经提取、过滤、浓缩、复溶后
经免疫亲和柱净化,进样后采用光化学行生器在线对黄曲霉毒素G1B1衍生,荧光检测器分析B1、B2
G1、G2、M1、M2六种黄曲霉毒素。结果在不同加标水平,不同肝脏回收率在70.2%6-94.1%之间,变异
系数为2.67%6~-12.90%6,检测低限为(0.16gkg。结论该方法的准确度与精密度较高,可以满足痕量分析
要求。
关键词:黄曲霉毒素;肝脏;高效液相色谱法;免疫亲和柱
Determination of six aflatoxins in animal liver by photochemical
derivative-high performance liquid chromatography combining
with immunoaffinity column clean-up
CHEN Dong-dong DA Han-xia PENG Tao, YAO Jia, WANG Xin,, ZHANG Jian-xin
LI Nan,,, WANG Xiong
(1. Chinese Academy ofinspection and uarantine, Beiing 100025, China; 2. Northwest Sci-fech University of A g7icultue
and Forestry, Yangling 712100 China; 3. Beijing Clovertech Limied Company, Beijing 100044, China)
ABSTRACT: Objcctive To develop a method for detecting six aflatoxins in chicken and pig liver
Methods Samples were cxtracted, filtcrcd, concentrated and reconstituted purified by Immunoaffinity
column, derivatived aflatoxin G B using photochemical derivatization unil online aller injection Ihen six
aflatoxins 13 132, (], (i, M, and M were analyzed by fluorescence detector. Results The recoveries of
samples with different spiking levels were 70.2%0-94.1%0 The RSD were 267-12.90%. Thc lowest limit of
quantification was 0.16 ug/kg. Conclusion This method is o[ high accuracy and precision, and can mee
the requirements for trace analysis
KEY WORDS: aflatoxins; liver; high performance liquid chromatography immunoaffinity column
基金项目:国家科技支撑计划项目(2012BAKO8BO1)
Iund: Supported by National Key R&1) Program(201213/
通讯作者:王雄,博士,高级工程师,主要研究方向为食品安全检测与超微量分析化学。T-mai: wang(@clovertek com
Corresponding author: WANG Xiong, Senior Enginccr, Beijing Clovcrtcch Limited Company. No56A South Strcct, Zhongguancun, Fangyuan
Mansion, Suite B0201, Beijing 100044, China. E-mail: wang(clovcrtck com