超声辅助双水相集成提取芒果核多酚工艺优化.pdf

第28卷第19期
农业工程学报
2012年10月
Transactions of the Chinese Society of Agricuitural Engineering
Oct.2012255
Optimization of ultrasound-assisted aqueous two-phase
extraction of polyphenols from mango seed kernel
Gao Yuntao, Wang Zhenfeng,, Luo Lijun 2, Dai Jianhui
ib,2, Li Ding
(1. School of Chemistry and Biotechnology, Yunnan University of Nationalities, Kunming 650031, China
2. Key Laboratory of Ethnic Medicine Resource Chemistry,
State Ethnic Afairs ommission and Ministry of Education, Kunming, 650500, China)
Abstract: In order to obtain the optimal conditions of ultrasound-assisted aqueous two-phase extraction of pol phenols
from mango seed kernel, a novel extraction method involving ultrasound-assisted extraction combined with
propyl-alcohol ammonium sul ate aqueous two-phase separation was developed for the extraction of polyphenols from
mango seed kernel. Response surface methodology, based on a five levels, four variables central composite designs was
employed to study the effect of extraction conditions on the extraction yield of total polyphenols. An extraction yield of
6.67% was achieved under the optimal extraction conditions of 59.7% of propyl-alcohol volume fraction, 23.4% f
ammonium sul ate investigated concentration, 27 0 min of extraction time and liquid/solid ratio of 23 m/g at ultrasonic
power of 400 W and temperature of 45C, which was agreement well with the optimal prediction value(6.82%). Total
polyphenols content in the extract of ultrasound-assisted aqueous two-phase extraction was higher than that of
conventional ultrasound-assisted extraction. The research indicates that the method of combining aqueous two-phase
separation with ultrasound-assisted extraction to improve the extraction of polyphenols is practicable. The research can
provide a reference for full utilization of mango seed kernel and the extraction technique of polyphenols
Key words: optimization, extraction, ultrasonics, polyphenols, mango seed kernel
doi:10.3969isSn.1002-6819.2012.19.034
CLC number: TS201
Document code: A
Article ID:1002-6819(2012)-190255-07
Gao Yuntao, Wang Zhenfeng, Luo Lijun, et al. Optimization of ultrasound-assisted aqueous two-phase extraction of
olyp enols from mango seed kernel ] Transactions of the Chinese Society of Agricultural Engineering(Transactions of
the CSAE), 2012, 28(19): 255-261.(in English with Chinese abstract)
高云涛,王振峰,罗利军,等.超声辅助双水相集成提取芒果核多酚工艺优化[.农业工程学报,2012,28(19)
255-261.
0 ntroduction
mpound of mango seed kernel is polypheno/ [iy, c
enzymes and dietary fibrelb-10. The main bioactiv
Mango (Mangifera indica L) is a tropical or that have desirable biological properties! ",such as
subtropical fruit that belongs to the family of strong antioxidant
Anacardiaceae. About 20%o of fruits are processed for
Ultrasound-assisted extraction has become a well
products such as pure, nectar, leather, pickles and established method in the processing of plant material
canned sitoli) Mango seed kernel is a major
due to its high efficiency, low energy and water
by-product during mango processing-). Mango seed consumption. Ultrasonic treatment is time saving and
kemel is not currently utilized for commercial purpose, enhance the extraction of phenolic compounds
it is discarded as a waste and becomes a source o