MFHPB-01-SUP.pdf

Published on the Food Directorates (Health Canadas) website at http:/www.hc-sc.gc.ca/food-aliment. Government of Canada Gouvernement du Canada Supplement to MFHPB-01October 2001HEALTH PRODUCTS AND FOOD BRANCHOTTAWASUPPLEMENT TO THE METHOD MFHPB-011. APPLICATIONThe following information is offered as a supplement to the method MFHPB-01, dated March 2001, andshould be used with this method. The purpose of this supplement is to provide additional information(such as source(s) of materials, critical steps, confirmation steps, or helpful hints etc.).2. MATERIALS AND SPECIAL EQUIPMENT1)Laminar air flow cabinet or biological safety cabinet, located in a clean environmentCarry out all microbiological analyses under a laminar flow cabinet or biological safety cabinet,located in a clean environment. The minimum requirements for a clean environment is alaboratory (or a portion of the laboratory) containing no open windows and designed sothat there are no direct drafts across the work area. The particle count of the laminar flow should not exceed a total of 100 particles of size 0.5micron or greater per cubic foot, (Class 100, U.S. Federal Standard 209B). A Class 10,000clean room is acceptable under the provisions detailed in section 8.1.3. A class 100,000 cleanroom has also proved to be satisfactory providing additional quality assurance measures asdetailed in section 8.1.3 are taken. 9. REFERENCES9.1Association of Official Analytical Chemists (AOAC). 1995. Chapter 21. BacteriologicalAnalytical Manual, Arlington, Virginia, USA.9.2Association of Official Analytical Chemists (AOAC). 1995, Supplement March 1997. OfficialMethods of Analysis of AOAC International 16th Edition, Gaithersburg, Maryland, USA.